EC Number |
---|
1.18.1.6 | - |
1.18.1.6 | adrenodoxin-Sepharose affinity column chromatography |
1.18.1.6 | ammonium sulfate precipitation, adrenodoxin-Sepharose 6B column chromatography and Sephadex G-100 gel filtration |
1.18.1.6 | DEAE-Fractogel column chromatography and adrenodoxin-Sepharose column chromatography |
1.18.1.6 | ion exchange chromatography and 2'-5'-ADP-Sepharose column chromatography |
1.18.1.6 | octylamino-Sepharose 4B column chromatography, Sephadex G- 100 gel filtration and DEAE-Biogel A column chromatography |
1.18.1.6 | recombinant enzyme from Escherichia coli to homogeneity |
1.18.1.6 | Sephadex G-100 gel filtration and DEAE-cellulose column chromatography |
1.18.1.6 | the tightly bound enzyme form is purified by sodium cholate solubilization, ammonium sulfate precipitation, DEAE-Sepharose column chromatography, 2,5-ADP-Sepharose column chromatography, and DEAE-Sepharose column chromatography. The loosely bound enzyme form is purified by ammonium sulfate precipitation, DEAE-cellulose column chromatography, 2,5-ADP-Sepharose column chromatography, and Sephadex G-25 gel filtration |