EC Number |
---|
1.14.15.4 | ammonium sulfate fractionation, ion-exchange chromatography |
1.14.15.4 | ammonium sulfate precipitation, octyl-Sepharose adsorption with cholate and phosphotidylcholine extraction |
1.14.15.4 | cholate extraction, hydroxyapatite HPLC |
1.14.15.4 | copurification of 11beta-/18-hydroxylase with 11beta-/19-hydroxylase, cholate extraction |
1.14.15.4 | copurification of 11beta-/18-hydroxylase, cholate extraction, ammonium sulfate precipitation, Tween extraction, octymine-Sepharose |
1.14.15.4 | copurification of 11beta-hydroxylase with 18-hydroxylase, ammonium sulfate, DEAE-chromatography |
1.14.15.4 | copurification of P-450 11beta-2 and 11beta-3, ammonium sulfate precipitation, octyl-Sepharose, separation of 2 isoforms by hydroxyapatite column connected to an FPLC system |
1.14.15.4 | Na-cholate extraction, ammonium sulfate precipitation, aniline-Sepharose chromatography, hydroxylapatite HPLC |
1.14.15.4 | native enzyme by anion exchange and hydrophobic interaction chromatography, and two additonal steps of anion exchange chromatography |
1.14.15.4 | octyl-Sepharose chromatography, cholate extraction |