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EC Number
Purification (Commentary)
1.14.11.65
recombinant full-length His6-tagged LSD1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
1.14.11.65
recombinant GST-tagged Smw1 from Escherichia coli strain Rosetta by glutathione affinity chromatography
1.14.11.65
recombinant N-terminally His-tagged enzyme by nickel affinity chromatography from Escherichia coli BL21(DE3), or by ammonium sulfate fractionation and anion exchange chromatography, recombinant N-terminally truncated GST-tagged LSD1 by glutathione affinity chromatography
1.14.11.65
Streptactin-Sepharose column chromatography and FLAG M2-agarose column chromatography
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