EC Number |
---|
1.12.99.6 | - |
1.12.99.6 | 467fold with a yield of 0.13%, to homogeneity, under anaerobic and reducing conditions using sodium dithionite as reducing agent, by ultrafiltration |
1.12.99.6 | ammonium sulfate precipitation and phenyl-Sepharose column chromatography |
1.12.99.6 | apparent homogeneity |
1.12.99.6 | by affinity chromatography, ca. 99% pure |
1.12.99.6 | by gel filtration, 55fold |
1.12.99.6 | by immobilized metal-affinity chromatography |
1.12.99.6 | cytosolic [NiFe] hydrogenase large subunit HyhL |
1.12.99.6 | DEAE 52 cellulose column chromatography |
1.12.99.6 | DEAE Sepharose column chromatography and Q Sepharose HiLoad column chromatography |