EC Number   |
Application |
Reference |
|---|
    2.3.2.2 | analysis |
analytical method for the selective determination and speciation of S-nitrosoglutathione and its metabolite S-nitrosocysteinylglycine, based on liquid chromatography separation coupled to on-line enzymatic hydrolysis of S-nitrosoglutathione by commercial GGT. The limit of quantitation for S-nitrosoglutathione and S-nitrosocysteinylglycine in plasma ultrafiltrate is 5 nM, with a precision of 1-6% within the 5-1500 nM dynamic linear range. The method is applied to evaluate the recovery of exogenous S-nitrosoglutathione after addition of aliquots to human plasma samples presenting with different total GGT activities. By inhibiting GGT activity in a time-dependent manner, the recovery of S-nitrosoglutathione is inversely correlated with plasmatic levels of endogenous GGT |
701918 |
| 2.3.2.2 | analysis |
assays for gamma-glutamyl transferase (GGT1) activity in blood are widely used in a clinical setting to measure tissue damage |
687030 |
| 2.3.2.2 | analysis |
gamma-glutamyltransferase can be used as a marker related with oxidative stress |
687000 |
| 2.3.2.2 | analysis |
GGT level is a good predictor of low coronary flow reserve at the receiver-operating characteristic curve |
688145 |
| 2.3.2.2 | analysis |
plasma layer stratification might occur in primary lithium-heparin tubes for a limited number of routine clinical chemistry tests, introducing a statistically significant bias in the measurement of gamma-glutamyltransferase, lactate dehydrogenase, triglycerides and C-reactive protein in the upper vs. the bottom section. When delayed testing is necessary for these parameters, plasma should be separated after centrifugation and appropriately mixed before delayed/repeated analysis or aliquoting |
686156 |
| 2.3.2.2 | analysis |
simple, robust and highly sensitive assay based on an alpha-phenylthio-containing glutathione peptide mimic gamma-glutamyl-D,L-phenylthioglycylglycine that eliminates thiophenol upon GGT-catalyzed hydrolysis of the c-glutamyl peptide bond |
702034 |
| 2.3.2.2 | diagnostics |
direct detection of GGT activity can provide critical information for the diagnosis of several pathologies. Elevated serum GGT levels are a general marker of diseases affecting the liver |
755785 |
| 2.3.2.2 | diagnostics |
the GGT enzyme is known as a diagnostic enzyme for liver and biliary related diseases |
-, 756619 |
| 2.3.2.2 | drug development |
development of 5-thiohistidine derivatives as therapeutics for GGT-positive tumors |
757548 |
| 2.3.2.2 | food industry |
development of an enzymatic method involving Bacillus licheniformis GGT (BlGGT) for the synthesis of gamma-glutamyl-L-leucine (gamma-Glu-Leu) from glutamine and leucine, since the mass production of Kokumi peptides from natural resources in high purity generally involves several tedious and inefficient operation processes. Parameter optimization of the BlGGT-catalyzed reaction for the biocatalytic synthesis of this Kokumi-imparting molecule |
756753 |
