EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
2.1.1.45 | -999 |
- |
more |
Michaelis-Menten kinetics |
742057, 743569 |
2.1.1.45 | -999 |
- |
more |
single turnover, stopped-flow, and steady-state kinetics |
741889 |
2.1.1.45 | 0.00068 |
- |
5,10-formyltetrahydropteroylheptaglutamate |
- |
485398 |
2.1.1.45 | 0.0007 |
- |
2'-deoxyuridylate |
mutant 362(T51S,K82Q,N171S) |
485433 |
2.1.1.45 | 0.00074 |
- |
5,10-methenyltetrahydropteroylpentaglutamate |
- |
485398 |
2.1.1.45 | 0.00085 |
- |
dUMP |
mutant enzyme A191K, at 37°C |
706625 |
2.1.1.45 | 0.00096 |
- |
dUMP |
mutant enzyme M190E, at 37°C |
706625 |
2.1.1.45 | 0.001 |
- |
2'-deoxyuridylate |
mutant Y258F |
485433 |
2.1.1.45 | 0.001 |
- |
methylenetetrahydropteroylgluamate |
mutant P254S |
485426 |
2.1.1.45 | 0.0011 |
- |
2'-deoxyuridylate |
mutant 302(T53S,Y258F) |
485433 |