EC Number   |
General Stability |
Reference |
|---|
    1.2.1.12 | 1% SDS, fully active |
287938 |
| 1.2.1.12 | 5 M, 0°C, 1 h, complete loss of activity, 5 mM NAD+ protects |
287966 |
| 1.2.1.12 | 5.0 M guanidine-HCl, rapid and irreversible inactivation at 30°C |
287970 |
| 1.2.1.12 | 8 M urea, 30°C, 0.05 M phosphate buffer, 50% loss of activity after 1 min |
287963 |
| 1.2.1.12 | 8 M urea, 40% inactivation |
287938, 287968 |
| 1.2.1.12 | 8.0 M urea, unusually resistant at 30°C, 40°C and 50°C. Rapid inactivation at 55°C and at 60°C |
287970 |
| 1.2.1.12 | acetylleucine chloromethyl ketone binds to GAPDH to modulate the conformation of the enzyme, the modified enzyme is susceptible to chymotrypsin-like protease activity, cleavage at TRp195-Arg196 |
654926 |
| 1.2.1.12 | alpha-crystallin accelerates the thermal inactivation of GAPDH, while GroEL does not affect thermal inactivation and denaturation of GAPDH |
713466 |
| 1.2.1.12 | binding to erythrocyte membranes stabilizes the enzyme at 4°C |
287950 |
| 1.2.1.12 | freezing in liquid nitrogen results in partial loss of activity |
287933 |
