EC Number   |
General Stability |
Reference |
|---|
   1.17.3.2 | heparin-Sepharose 6B-bound enzyme, half-life: 120 h and 67 h at 4°C and 20°C respectively, free enzyme, 61 h and 45 h at 4°C and 67°C respectively |
644628 |
| 1.17.3.2 | low operational stability by immobilization |
644649 |
| 1.17.3.2 | phosphate stabilizes |
644645 |
| 1.17.3.2 | rat liver enzyme is unstable as dehydrogenase and is gradually converted to oxidase |
644580 |
| 1.17.3.2 | stable for more than 1 year with alternate freezing and thawing |
644621 |
| 1.17.3.2 | the enzyme from animal tissues can be interconverted to EC 1.1.1.204, that from liver exists in vivo mainly as the dehydrogenase form, but can be converted into the oxidase form by storage at -20°C, by treatment with proteolytic enzymes or with organic solvents, or by thiol reagents such as Cu2+, N-ethylmaleimide or 4-hydroxymercuribenzoate, the effect of the thiol reagents can be reversed by thiols such as 1,4-dithioerythritol, in other animal tissues the enzyme exists almost entirely as EC 1.1.3.22 but can be converted into the dehydrogenase form by 1,4-dithioerythritol |
644582, 644640, 644643 |
| 1.17.3.2 | the xanthine dehydrogenase form can be obtained through incubation of xanthine oxidase with sulfhydryl reducing reagents |
390370 |
