EC Number   |
Activating Compound |
Reference |
|---|
    1.23.5.1 | digalactosyldiacylglyceride |
0.0067 mM, supports slow but nevertheless complete to nearly complete de-epoxidation |
676718 |
| 1.23.5.1 | Lipid |
the enzyme requires lipid inverted hexagonal structures for activity |
658065 |
| 1.23.5.1 | monogalactosyldiacylglyceride |
0.01 mM, supports rapid and complete de-epoxidation |
676718 |
| 1.23.5.1 | monogalactosyldiacylglycerol |
0.0116 mM is needed to achieve saturation of the de-epoxidation reaction |
672021 |
| 1.23.5.1 | monogalactosyldiacylglycerol |
required for an active enzyme. Optimal ratio of monogalactosyldiacylglycerol to the substrate violaxanthin is 28:1 |
657634 |
| 1.23.5.1 | more |
effect of fluidity of hydrophobic fraction of inverted micelles composed of different types of lipids on enzyme activity, best activation by monogalactosyldiacylglycerol |
723818 |
| 1.23.5.1 | more |
sequence analysis and site directed mutagenesis show that 12 cysteines are conserved in most photosynthetic eukaryotes and they are also fundamental for the activity of VDE from Arabidopsis thaliana, being involved in the formation of several structural/regulatory disulfide bonds |
744904 |
| 1.23.5.1 | more |
the gel to liquid-crystalline phase transition in the single lipid component systems strongly enhances both the solubilization of the xanthophyll cycle pigment violaxanthin in the membrane and the activity of the violaxanthin de-epoxidase. This phase transition has a significantly stronger impact on violaxanthin de-epoxidase activity than the transition from the liquid-crystalline phase to the inverted hexagonal phase. Especially at higher temperatures increased violaxanthin de-epoxidase reaction rates are detected in the presence of the liquid-crystalline phase compared to those in the presence of HII phase forming lipids. The HII phase is better suited to maintain high violaxanthin de-epoxidase activities at lower temperatures |
685365 |
| 1.23.5.1 | more |
VDE activation is triggered by a pH reduction in the thylakoids lumen occurring under saturating light, mechanism of the VDE activation, overview. VDE activation relies on a robust and redundant network, in which the four residues D98, D117, H168 and D206 play a major role |
726294 |
| 1.23.5.1 | phosphatidylcholine |
supports slow and incomplete de-epoxidation |
676718 |
