3.1.1.7	1,1-dimethyl-4-acetylthiomethylpiperidinium + H2O	i.e. MATP+, paper spray mass spectrometry is used to monitor the activity of acetylcholinesterase, both in-solution and on modified hydrophobic paper surface. Hydrophobic paper surfaces are prepared using vaporized trichloro(3,3,3-trifluoropropyl)silane. In both approaches, mixtures of diluted human whole blood with and without inhibitor ethyl ((2-[bis(propan-2-yl)amino]ethyl)sulfanyl)(methyl)phosphonate (VX) are mixed with a non-endogenous AChE specific substrate, 1,1-dimethyl-4-acetylthiomethylpiperidinium (MATP+). Formation of the cleaved MATP+ product is monitored over time and compared to MATP+ to determine relative AChE activity. This on-substrate assay is effective at determining AChE activity and identifying the toxicant, while determination of AChE activity in-solution proceeds at a slower rate. Method evaluation, overview	Homo sapiens	?	-	?	446522	
3.1.1.7	1-naphthyl acetate + H2O	a chromogenic substrate for detection of erythrocyte acetylcholinesterase activity	Homo sapiens	1-naphthol + acetate	-	?	12515	
3.1.1.7	2,6-dichloro-3'-methylindophenylacetate + H2O	-	Neptunea eulimata	2,6-dichloro-3'-methylindophenol + acetate	-	?	364393	
3.1.1.7	2,6-dichloro-3'-methylindophenylacetate + H2O	weak substrate	Homo sapiens	2,6-dichloro-3'-methylindophenol + acetate	-	?	364393	
3.1.1.7	2,6-dichlorophenolindophenol acetate + H2O	2,6-dichlorophenolindophenol acetate computational molecular docking study using the enzyme's crystal structure, PDB ID 4YE7	Homo sapiens	2,6-dichlorophenolindophenol + acetate	-	?	446624	
3.1.1.7	2,6-dichlorophenolindophenylacetate + H2O	-	Homo sapiens	2,6-dichlorophenolindophenol + acetate	-	?	364391	
3.1.1.7	2,6-dichlorophenolindophenylacetate + H2O	-	Neptunea eulimata	2,6-dichlorophenolindophenol + acetate	-	?	364391	
3.1.1.7	2-methoxyacetylthiocholine + H2O	recombinant enzyme shows about 12% activity compared to acetylthiocholine	Homo sapiens	thiocholine + 2-methoxyacetate	-	?	416140	
3.1.1.7	2-methoxyacetylthiocholine + H2O	recombinant enzyme shows about 65% activity compared to acetylthiocholine	Lucilia cuprina	thiocholine + 2-methoxyacetate	-	?	416140	
3.1.1.7	3',5'-dichloro-2'-methylindophenylacetate + H2O	weak substrate	Homo sapiens	3',5'-dichloro-2'-methylindophenol + acetate	-	?	364394