3.1.3.100	4-nitrophenyl phosphate + H2O	100% activity	4-nitrophenol + phosphate	-	?	73771	
3.1.3.100	FMN + H2O	73% activity compared to 4-nitrophenyl phosphate	?	-	?	93350	
3.1.3.100	L-phosphotyrosine + H2O	32% activity compared to 4-nitrophenyl phosphate	phosphate + L-tyrosine	-	?	447669	
3.1.3.100	additional information	less than 1% activity with pyridoxal 5'-phosphate, beta-glycerophosphate, D-glucose 6-phosphate, D-fructose 1,6-diphosphate, inosine 5'-monophosphate, or adenosine 5'-monophosphate	?	-	?	89	
3.1.3.100	thiamine + phosphate	-	thiamine phosphate + H2O	-	?	434819	
3.1.3.100	thiamine diphosphate + H2O	preferred substrate	thiamine phosphate + phosphate	-	?	434820	
3.1.3.100	thiamine phosphate + H2O	-	thiamine + phosphate	-	?	418098	
3.1.3.100	thiamine phosphate + H2O	18% activity compared to 4-nitrophenyl phosphate	thiamine + phosphate	-	?	418098