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Literature summary for 3.1.1.7 extracted from

  • Mirzajani, F.; Motevalli, S.M.; Jabbari, S.; Ranaei Siadat, S.O.; Sefidbakht, Y.
    Recombinant acetylcholinesterase purification and its interaction with silver nanoparticle (2017), Protein Expr. Purif., 136, 58-65 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His-tagged enzyme in eukaryote cells using the baculovirus transfection system Drosophila melanogaster

Inhibitors

Inhibitors Comment Organism Structure
additional information the interaction between AChE and silver nanoparticles (AgNPs) leads not only to a decrease in AChE activity, but also to a reduction in the crystallinity and stability of AgNPs. The circular dichroism demonstrates that the secondary structure of AChE also declines after 30 min of incubation with AgNPs at 37°C. Smaller AgNPs result in size increments after interaction with enzymes, while the larger ones show size decrements Drosophila melanogaster

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster P07140
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme by procainamide affinity chromatography and desalting gel filtration Drosophila melanogaster

Synonyms

Synonyms Comment Organism
AChE
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Drosophila melanogaster

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Drosophila melanogaster

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Drosophila melanogaster