Cloned (Comment) | Organism |
---|---|
gene Tcace1, DNA and amino acid sequence determination and analysis, recombinant expression of isozyme TcAChE1 in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system, quantitative reverse transcription PCR enzyme expression analysis | Aphis citricidus |
gene Tcace2, DNA and amino acid sequence determination and analysis, recombinant expression of isozyme TcAChE2 in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system, quantitative reverse transcription PCR enzyme expression analysis | Aphis citricidus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | silencing of isozyme Tcace1 by RNA interference significantly increases the susceptibility of Toxoptera citricidus to malathion and carbaryl, silencing of isozyme Tcace1 results in a highly increased mortality rate. The specific enzyme activity decreases after silencing of Tcace1 | Aphis citricidus |
additional information | silencing of isozyme Tcace2 by RNA interference significantly increases the susceptibility of Toxoptera citricidus to malathion and carbaryl, silencing of isozyme Tcace2 results in an increased mortality rate. The specific enzyme activity decreases after silencing of Tcace2 | Aphis citricidus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Aldicarb | - |
Aphis citricidus | |
BW284c51 | - |
Aphis citricidus | |
Carbaryl | - |
Aphis citricidus | |
carbofuran | - |
Aphis citricidus | |
eserine | - |
Aphis citricidus | |
isoprocarb | - |
Aphis citricidus | |
malathion | - |
Aphis citricidus | |
additional information | greater sensitivity of recombinant isozyme TcAChE1 to the 10 tested insecticides compared to isozyme TcAChE2; greater sensitivity of recombinant isozyme TcAChE1 to the 10 tested insecticides compared to isozyme TcAChE2 | Aphis citricidus | |
omethoate | - |
Aphis citricidus | |
paraoxon-ethyl | - |
Aphis citricidus | |
propoxur | - |
Aphis citricidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics. In comparison, isozyme TcAChE1 has a lower Michaelis-Menten constant value and a higher maximal reaction velocity than recombinant isozyme TcAChE2, indicating a higher affinity for substrates and greater catalytic efficiency, respectively | Aphis citricidus | |
0.016 | - |
propionylthiocholine | pH 7.3, 37°C, recombinant enzyme | Aphis citricidus | |
0.026 | - |
acetylthiocholine | pH 7.3, 37°C, recombinant enzyme | Aphis citricidus | |
0.056 | - |
butyrylthiocholine | pH 7.3, 37°C, recombinant enzyme | Aphis citricidus | |
0.062 | - |
propionylthiocholine | pH 7.3, 37°C, recombinant enzyme | Aphis citricidus | |
0.065 | - |
acetylthiocholine | pH 7.3, 37°C, recombinant enzyme | Aphis citricidus | |
0.206 | - |
butyrylthiocholine | pH 7.3, 37°C, recombinant enzyme | Aphis citricidus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylcholine + H2O | Aphis citricidus | - |
choline + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aphis citricidus | A0A0X9E4F6 | Aphis citricidus | - |
Aphis citricidus | A0A0X9FVC0 | Aphis citricidus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylcholine + H2O | - |
Aphis citricidus | choline + acetate | - |
? | |
acetylthiocholine + H2O | - |
Aphis citricidus | thiocholine + acetate | - |
? | |
butyrylthiocholine + H2O | - |
Aphis citricidus | thiocholine + butyrate | - |
? | |
propionylthiocholine + H2O | - |
Aphis citricidus | thiocholine + propionate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acetylcholinesterase 2 | - |
Aphis citricidus |
AChE | - |
Aphis citricidus |
AChE_1 | - |
Aphis citricidus |
AChE_2 | - |
Aphis citricidus |
Tcace1 | - |
Aphis citricidus |
Tcace2 | - |
Aphis citricidus |
TcAChE1 | - |
Aphis citricidus |
TcAChE2 | - |
Aphis citricidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Aphis citricidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
assay at | Aphis citricidus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Aphis citricidus |
General Information | Comment | Organism |
---|---|---|
malfunction | silencing of Tcace1 and Tcace2 by RNA interference significantly increases the susceptibility of Toxoptera citricidus to malathion and carbaryl, silencing of isozyme Tcace1 results in a higher mortality rate than silencing of isozyme Tcace2. Additionally, the specific enzyme activity decreases more after silencing Tcace1 than after silencing Tcace2 | Aphis citricidus |