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Literature summary for 3.1.1.7 extracted from

  • Mou, X.; Yuan, G.R.; Jiang, H.B.; Liu, Z.; Wang, J.J.
    Functional characterization of two acetylcholinesterase genes in the brown citrus aphid, Aphis (Toxoptera) citricidus (Kirkaldy), using heterologous expression and RNA interference (2017), Pestic. Biochem. Physiol., 138, 76-83 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene Tcace1, DNA and amino acid sequence determination and analysis, recombinant expression of isozyme TcAChE1 in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system, quantitative reverse transcription PCR enzyme expression analysis Aphis citricidus
gene Tcace2, DNA and amino acid sequence determination and analysis, recombinant expression of isozyme TcAChE2 in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system, quantitative reverse transcription PCR enzyme expression analysis Aphis citricidus

Protein Variants

Protein Variants Comment Organism
additional information silencing of isozyme Tcace1 by RNA interference significantly increases the susceptibility of Toxoptera citricidus to malathion and carbaryl, silencing of isozyme Tcace1 results in a highly increased mortality rate. The specific enzyme activity decreases after silencing of Tcace1 Aphis citricidus
additional information silencing of isozyme Tcace2 by RNA interference significantly increases the susceptibility of Toxoptera citricidus to malathion and carbaryl, silencing of isozyme Tcace2 results in an increased mortality rate. The specific enzyme activity decreases after silencing of Tcace2 Aphis citricidus

Inhibitors

Inhibitors Comment Organism Structure
Aldicarb
-
Aphis citricidus
BW284c51
-
Aphis citricidus
Carbaryl
-
Aphis citricidus
carbofuran
-
Aphis citricidus
eserine
-
Aphis citricidus
isoprocarb
-
Aphis citricidus
malathion
-
Aphis citricidus
additional information greater sensitivity of recombinant isozyme TcAChE1 to the 10 tested insecticides compared to isozyme TcAChE2; greater sensitivity of recombinant isozyme TcAChE1 to the 10 tested insecticides compared to isozyme TcAChE2 Aphis citricidus
omethoate
-
Aphis citricidus
paraoxon-ethyl
-
Aphis citricidus
propoxur
-
Aphis citricidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics. In comparison, isozyme TcAChE1 has a lower Michaelis-Menten constant value and a higher maximal reaction velocity than recombinant isozyme TcAChE2, indicating a higher affinity for substrates and greater catalytic efficiency, respectively Aphis citricidus
0.016
-
propionylthiocholine pH 7.3, 37°C, recombinant enzyme Aphis citricidus
0.026
-
acetylthiocholine pH 7.3, 37°C, recombinant enzyme Aphis citricidus
0.056
-
butyrylthiocholine pH 7.3, 37°C, recombinant enzyme Aphis citricidus
0.062
-
propionylthiocholine pH 7.3, 37°C, recombinant enzyme Aphis citricidus
0.065
-
acetylthiocholine pH 7.3, 37°C, recombinant enzyme Aphis citricidus
0.206
-
butyrylthiocholine pH 7.3, 37°C, recombinant enzyme Aphis citricidus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetylcholine + H2O Aphis citricidus
-
choline + acetate
-
?

Organism

Organism UniProt Comment Textmining
Aphis citricidus A0A0X9E4F6 Aphis citricidus
-
Aphis citricidus A0A0X9FVC0 Aphis citricidus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylcholine + H2O
-
Aphis citricidus choline + acetate
-
?
acetylthiocholine + H2O
-
Aphis citricidus thiocholine + acetate
-
?
butyrylthiocholine + H2O
-
Aphis citricidus thiocholine + butyrate
-
?
propionylthiocholine + H2O
-
Aphis citricidus thiocholine + propionate
-
?

Synonyms

Synonyms Comment Organism
acetylcholinesterase 2
-
Aphis citricidus
AChE
-
Aphis citricidus
AChE_1
-
Aphis citricidus
AChE_2
-
Aphis citricidus
Tcace1
-
Aphis citricidus
Tcace2
-
Aphis citricidus
TcAChE1
-
Aphis citricidus
TcAChE2
-
Aphis citricidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Aphis citricidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
assay at Aphis citricidus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information inhibition kinetics Aphis citricidus

General Information

General Information Comment Organism
malfunction silencing of Tcace1 and Tcace2 by RNA interference significantly increases the susceptibility of Toxoptera citricidus to malathion and carbaryl, silencing of isozyme Tcace1 results in a higher mortality rate than silencing of isozyme Tcace2. Additionally, the specific enzyme activity decreases more after silencing Tcace1 than after silencing Tcace2 Aphis citricidus