Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetamiprid | complete inhibition | Heterorhabditis bacteriophora | |
carbofuran | complete inhibition | Heterorhabditis bacteriophora | |
malathion | non-competitive inhibition | Heterorhabditis bacteriophora | |
methomyl | complete inhibition | Heterorhabditis bacteriophora | |
additional information | AChE isoenzymes, AChEAII and AChEBI isolated from Heterorhabditid bacteriophora EM2 strain, show insensitivity to inhibition by insecticides in a descending order: methomyl, carbofuran, acetamiprid, oxamyl, malathion. The two AChE isoforms are several folds less sensitive to inhibition by methomyl and carbofuran compared to those previously reported for other insect species. Except for malathion, all the insecticides competitively inhibit Heterorhabditid bacteriophora AChEs with Ki values ranging from 0.10 to 15 mM | Heterorhabditis bacteriophora | |
oxamyl | complete inhibition | Heterorhabditis bacteriophora |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylcholine + H2O | Heterorhabditis bacteriophora | - |
choline + acetate | - |
? | |
acetylcholine + H2O | Heterorhabditis bacteriophora EM2 | - |
choline + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Heterorhabditis bacteriophora | - |
- |
- |
Heterorhabditis bacteriophora EM2 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
200 | - |
total activity of AChE in nematodes of strain EM2, pH 8.5, 37°C | Heterorhabditis bacteriophora |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylcholine + H2O | - |
Heterorhabditis bacteriophora | choline + acetate | - |
? | |
acetylcholine + H2O | - |
Heterorhabditis bacteriophora EM2 | choline + acetate | - |
? | |
acetylthiocholine + H2O | - |
Heterorhabditis bacteriophora | thiocholine + acetate | - |
? | |
acetylthiocholine + H2O | - |
Heterorhabditis bacteriophora EM2 | thiocholine + acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AChE | - |
Heterorhabditis bacteriophora |
AChEAII | - |
Heterorhabditis bacteriophora |
AChEBI | - |
Heterorhabditis bacteriophora |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
isozymes AChEAII and AChEBI | Heterorhabditis bacteriophora |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
isozymes AChEAII and AChEBI | Heterorhabditis bacteriophora |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics analysis | Heterorhabditis bacteriophora | |
0.063 | - |
malathion | pH 8.5, 37°C, isozyme AChEAII | Heterorhabditis bacteriophora | |
0.075 | - |
malathion | pH 8.5, 37°C, isozyme AChEBI | Heterorhabditis bacteriophora | |
0.1 | - |
oxamyl | pH 8.5, 37°C, isozyme AChEBI | Heterorhabditis bacteriophora | |
0.12 | - |
oxamyl | pH 8.5, 37°C, isozyme AChEAII | Heterorhabditis bacteriophora | |
0.75 | - |
acetamiprid | pH 8.5, 37°C, isozyme AChEBI | Heterorhabditis bacteriophora | |
0.85 | - |
acetamiprid | pH 8.5, 37°C, isozyme AChEAII | Heterorhabditis bacteriophora | |
1.25 | - |
carbofuran | pH 8.5, 37°C, isozyme AChEBI | Heterorhabditis bacteriophora | |
2.5 | - |
carbofuran | pH 8.5, 37°C, isozyme AChEAII | Heterorhabditis bacteriophora | |
12.5 | - |
methomyl | pH 8.5, 37°C, isozyme AChEBI | Heterorhabditis bacteriophora | |
15 | - |
methomyl | pH 8.5, 37°C, isozyme AChEAII | Heterorhabditis bacteriophora |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.087 | - |
pH 8.5, 37°C, isozyme AChEBI | Heterorhabditis bacteriophora | malathion | |
0.11 | - |
pH 8.5, 37°C, isozyme AChEAII | Heterorhabditis bacteriophora | malathion | |
0.16 | - |
pH 8.5, 37°C, isozyme AChEBI | Heterorhabditis bacteriophora | oxamyl | |
0.21 | - |
pH 8.5, 37°C, isozyme AChEAII | Heterorhabditis bacteriophora | oxamyl | |
1.23 | - |
pH 8.5, 37°C, isozyme AChEBI | Heterorhabditis bacteriophora | acetamiprid | |
1.4 | - |
pH 8.5, 37°C, isozyme AChEBI | Heterorhabditis bacteriophora | carbofuran | |
1.64 | - |
pH 8.5, 37°C, isozyme AChEAII | Heterorhabditis bacteriophora | acetamiprid | |
2.57 | - |
pH 8.5, 37°C, isozyme AChEAII | Heterorhabditis bacteriophora | carbofuran | |
18 | - |
pH 8.5, 37°C, isozyme AChEBI | Heterorhabditis bacteriophora | methomyl | |
23 | - |
pH 8.5, 37°C, isozyme AChEAII | Heterorhabditis bacteriophora | methomyl |