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Literature summary for 3.1.1.7 extracted from

  • Liu, N.; Che, J.; Lai, D.; Wen, J.; Xu, H.
    High-level expression and purification of Plutella xylostella acetylcholinesterase in Pichia pastoris and its potential application (2017), J. Integr. Agric., 16, 1358-1366 .
No PubMed abstract available
Search for more literature for 3.1.1.7

Cloned(Commentary)

Cloned (Comment) Organism
gene ace, DNA and amino acid sequence determination and analysis, no polyadenylation signal (AATAAA) was found within the 3'-UTR, although a 14-bp span of poly (A) nucleotides is detected at its end, sequence comparisons, subcloning in Escherichia coli strain JM109, recombinant overexpression of enzyme AChE2 in Pichia pastoris strain GS115 using the methanol-inducible AOX1 promoter Plutella xylostella

Inhibitors

Inhibitors Comment Organism Structure
methamidophos
-
 Plutella xylostella
additional information the recombinant enzyme is sensitive to inhibition by methamidophos and pirimicarb, the calculated IC50 values of the two pesticides are 0.357 and 0.888 mg/l, respectively, and the calculated IC70 values are 0.521 and 0.839 mg/l, respectively Plutella xylostella
pirimicarb
-
 Plutella xylostella

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetylcholine + H2O Plutella xylostella
-
 choline + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Plutella xylostella Q8MZL2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme AChE2 12.1fold from Pichia pastoris strain GS115 by ammonium sulfate fractionation, procainamide affinity chromatography Plutella xylostella

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
448.3
-
 purified recombinant enzyme, pH and temperature not specified in the publication Plutella xylostella

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylcholine + H2O
-
 Plutella xylostella choline + acetate
-
 ?
acetylthiocholine + H2O
-
 Plutella xylostella thiocholine + acetate
-
 ?

Subunits

Subunits Comment Organism
? x * 67000, recombinant enzyme, SDS-PAGE Plutella xylostella

Synonyms

Synonyms Comment Organism
ACE
-
 Plutella xylostella
acetylcholinesterase 2
-
 Plutella xylostella
AChE2
-
 Plutella xylostella

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information inhibition kinetics Plutella xylostella