Cloned (Comment) | Organism |
---|---|
gene ace1, cloning from RNA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, Prace1 is distributed on genomic scaffold 156, Prace1 consists of three exons and two introns, quantitative real-time PCR expression analysis | Pieris rapae |
gene ace2, cloning from RNA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, Prace2 is distributed on genomic scaffold 430, Prace2 consists of six exons and five introns, quantitative real-time PCR expression analysis | Pieris rapae |
Protein Variants | Comment | Organism |
---|---|---|
G324A | naturally occuring mutation, conserved, associated with insecticide insensitivity | Pieris rapae |
S291G | naturally occuring mutation, conserved, associated with insecticide insensitivity | Pieris rapae |
S431F | naturally occuring mutation, conserved, associated with insecticide insensitivity | Pieris rapae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | isozyme PrAChE1 has a predicted signal peptide at its N-terminus, suggesting that the protein might be secreted into the extracellular fluid | Pieris rapae | - |
- |
extracellular | isozyme PrAChE2 has a predicted signal peptide at their N-terminus, suggesting that the protein might be secreted into the extracellular fluid | Pieris rapae | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pieris rapae | A0A1U9X1Z7 | i.e. Artogeia rapae, captured from an experimental cabbage (Brassica pekinensis) field in Anhui Agricultural University, Hefei, Anhui, China in May 2017 | - |
Pieris rapae | A0A385HWF0 | i.e. Artogeia rapae, captured from an experimental cabbage (Brassica pekinensis) field in Anhui Agricultural University, Hefei, Anhui, China in May 2017 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
head | larval | Pieris rapae | - |
larva | fifth-instar larval stage | Pieris rapae | - |
additional information | isozymes Prace1 and Prace2 are highly expressed at the fifth-instar larval stage and in the larval head, and the transcriptional levels of Prace1 are significantly higher than those of Prace2 in all of the tested life stages and tissues, expression profiles of the two Prace genes at different developmental stages, overview | Pieris rapae | - |
Subunits | Comment | Organism |
---|---|---|
? | x * 71700, about, isozyme PrAChE2, sequence calculation | Pieris rapae |
? | x * 78400, about, isozyme PrAChE1, sequence calculation | Pieris rapae |
Synonyms | Comment | Organism |
---|---|---|
AcE1 | - |
Pieris rapae |
ACE2 | - |
Pieris rapae |
AChE | - |
Pieris rapae |
Prace1 | - |
Pieris rapae |
Prace2 | - |
Pieris rapae |
PrAChE1 | - |
Pieris rapae |
PrAChE2 | - |
Pieris rapae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pieris rapae | isozyme PrAChE1, sequence calculation | - |
5.3 |
Pieris rapae | isozyme PrAChE1, sequence calculation | - |
6.1 |
General Information | Comment | Organism |
---|---|---|
evolution | isozymes PrAChE1 and PrAChE2 both have features typical of AChEs, including the catalytic triad, choline-binding sites, an oxyanion hole, an acyl pocket, a peripheral anionic subsite, an FGESAG motif and 14 conserved aromatic amino acids. Phylogenetic analysis shows that Prace1 and Prace2 are clustered into two distinct groups: ace1 and ace2, respectively | Pieris rapae |
physiological function | acetylcholinesterases (AChEs) are essential for the hydrolysis of the neurotransmitter acetylcholine and play crucial roles in the termination of neurotransmission | Pieris rapae |