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Literature summary for 3.1.1.7 extracted from

  • Pohanka, M.; Holas, O.
    Evaluation of 2,6-dichlorophenolindophenol acetate as a substrate for acetylcholinesterase activity assay (2015), J. Enzyme Inhib. Med. Chem., 30, 796-799 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene ACHE, recombinant expression in HEK-293 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2,6-dichlorophenolindophenol causes substrate inhibition at concentrations above 0.25 mM Homo sapiens
acetylthiocholine substrate inhibition at high concentrations Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.038
-
2,6-dichlorophenolindophenol recombinant enzyme, pH and temperature not specified in the publication Homo sapiens
0.18
-
acetylthiocholine recombinant enzyme, pH and temperature not specified in the publication Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetylcholine + H2O Homo sapiens
-
choline + acetate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P22303
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation recombinant enzyme expressed in HEK-293 cells Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,6-dichlorophenolindophenol acetate + H2O 2,6-dichlorophenolindophenol acetate computational molecular docking study using the enzyme's crystal structure, PDB ID 4YE7 Homo sapiens 2,6-dichlorophenolindophenol + acetate
-
?
acetylcholine + H2O
-
Homo sapiens choline + acetate
-
?
acetylthiocholine + H2O substrate is acetylthiocholine iodide Homo sapiens thiocholine + acetate
-
?
additional information the acetylcholinesterase (AChE) enzyme activity is detected using the 5,5'-dithio-bis(2-nitrobenzoic acid)-based colorimetric assay with measurement of the absorbance at 412 nm. 2,6-Dichloroindophenol acetate is performed as a chromogenic substrate also suitable for AChE activity examination Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
AChE
-
Homo sapiens