Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.7 extracted from

  • Carmany, D.O.; Mach, P.M.; Rizzo, G.M.; Dhummakupt, E.S.; McBride, E.M.; Sekowski, J.W.; Benton, B.; Demond, P.S.; Busch, M.W.; Glaros, T.
    On-substrate enzymatic reaction to determine acetylcholinesterase activity in whole blood by paper spray mass spectrometry (2018), J. Am. Soc. Mass Spectrom., 29, 2436-2442 .
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetylcholine + H2O Homo sapiens
-
choline + acetate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P22303
-
-

Source Tissue

Source Tissue Comment Organism Textmining
blood
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylcholine + H2O
-
Homo sapiens choline + acetate
-
?
acetylthiocholine + H2O
-
Homo sapiens thiocholine + acetate
-
?
1,1-dimethyl-4-acetylthiomethylpiperidinium + H2O i.e. MATP+, paper spray mass spectrometry is used to monitor the activity of acetylcholinesterase, both in-solution and on modified hydrophobic paper surface. Hydrophobic paper surfaces are prepared using vaporized trichloro(3,3,3-trifluoropropyl)silane. In both approaches, mixtures of diluted human whole blood with and without inhibitor ethyl ((2-[bis(propan-2-yl)amino]ethyl)sulfanyl)(methyl)phosphonate (VX) are mixed with a non-endogenous AChE specific substrate, 1,1-dimethyl-4-acetylthiomethylpiperidinium (MATP+). Formation of the cleaved MATP+ product is monitored over time and compared to MATP+ to determine relative AChE activity. This on-substrate assay is effective at determining AChE activity and identifying the toxicant, while determination of AChE activity in-solution proceeds at a slower rate. Method evaluation, overview Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
AChE
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens