Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged enzyme ClSChE in Spodoptera frugiperda SF9 cells via transfection using the baculovirus system | Cimex lectularius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics, recombinant enzyme | Cimex lectularius | |
0.0000569 | - |
Acetylcholine | pH 8.0, 25°C, recombinant enzyme | Cimex lectularius |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secretion of monomeric ClSChE through the proboscis during feeding | Cimex lectularius | - |
- |
additional information | isozyme ClSChE exists as two types, a dimer and a monomer, and neither is membrane-anchored, the soluble monomers of ClSChE are connected by a disulfide bond to form a dimer | Cimex lectularius | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylcholine + H2O | Cimex lectularius | - |
choline + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cimex lectularius | - |
Florida strain | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme ClSChE from Spodoptera frugiperda SF9 cells by affinity chromatography | Cimex lectularius |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
salivary gland | the common bed bug, Cimex lectularius, possesses a cholinesterase expressed exclusively in the salivary gland (ClSChE), molecular forms and tissue distribution patterns of ClSChE, immunohistochemical analysis confirms that ClSChE is expressed in the epithelial cells of both the salivary gland and the duct. ClSChE signal is slightly higher at the apical region of the main salivary gland than at the proximal part, and some ClSChE appears as aggregates dispersed inside the cytosol. In gland ducts, ClSChE staining is mainly localized in duct cells at the junction connecting to the main salivary gland. The salivary gland cells and some duct cells likely produce and secrete soluble ClSChE | Cimex lectularius | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylcholine + H2O | - |
Cimex lectularius | choline + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 130000, SDS-PAGE, salivary gland enzyme ClSChE exists as a soluble monomeric form or a soluble dimeric form connected by a disulfide bridge | Cimex lectularius |
monomer | 1 * 64000, SDS-PAGE, salivary gland enzyme ClSChE exists as a soluble monomeric form or a soluble dimeric form connected by a disulfide bridge, 1 * 54000, recombinant His-tagged enzyme, SDS-PAGE | Cimex lectularius |
Synonyms | Comment | Organism |
---|---|---|
ClSChE | - |
Cimex lectularius |
salivary gland-specific cholinesterase | - |
Cimex lectularius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Cimex lectularius |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00008 | - |
Acetylcholine | pH 8.0, 25°C, recombinant enzyme | Cimex lectularius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Cimex lectularius |
General Information | Comment | Organism |
---|---|---|
evolution | the bed bug uniquely possesses three genes encoding different cholinesterase (ChE) types [AChE1, AChE2 and salivary gland-specific cholinesterase (SChE)]. According to phylogenetic analysis, all three bed bug ChEs are categorized into a large clade of invertebrate ChEs. ClAChE1 is classified into the insect AChE1-type clade, whereas ClAChE2 is categorized into the insect AChE2-type clade. ClSChE is categorized into the clade containing the nematode and arachnid AChE1, which is closely located in an insect AChE1-type clade | Cimex lectularius |
physiological function | salivary gland enzyme ClSChE functions virtually as an acetylcholine-sequestering protein by having a very strong affinity to acetylcholine but an extremely long turnover time. Given that ACh regulates a wide variety of host physiologies, the tentative roles of ClSChE in blood vessel constriction and itch/pain regulation in the host are analyzed | Cimex lectularius |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.41 | - |
Acetylcholine | pH 8.0, 25°C, recombinant enzyme | Cimex lectularius |