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Literature summary for 3.1.1.7 extracted from

  • Mohamed, M.A.; Mahdy, e.l.-.S.M.; Ghazy, A.E.; Ibrahim, N.M.; El-Mezayen, H.A.; Ghanem, M.M.
    The activity of detoxifying enzymes in the infective juveniles of Heterorhabditis bacteriophora strains purification and characterization of two acetylcholinesterases (2016), Comp. Biochem. Physiol. C, 180, 11-22 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
acetylthiocholine bothe isozymes show substrate inhibition at 10 mM acetylthiocholine Heterorhabditis bacteriophora
BW284c51 specific and competitive inhibitor, time course of inhibition for the isozymes AChEA and AChEB, inactivation of both within 30 min at 0.6 mM BW284C51 Heterorhabditis bacteriophora
eserine nonspecific and competitive inhibitor, time course of inhibition for the isozymes AChEA and AChEB, 10% and 30% activity remain after 60 min at 1.0 mM eserine, respectively Heterorhabditis bacteriophora
Hg2+ AChEA and AChEB are completely inhibited by 1 mM of Hg2+ Heterorhabditis bacteriophora
K+ AChEA and AChEB lose 80% actiivty at 1 mM of K+ Heterorhabditis bacteriophora
additional information both isoenzymes are not affected by tetra(monoinopropyl)diphosphotetramide (isoOMPA) at high concentrations up to 50 mM Heterorhabditis bacteriophora
Ni2+ AChEA and AChEB are completely inhibited by 1 mM of Ni2+ Heterorhabditis bacteriophora

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics and Lineweaver-Burk plots Heterorhabditis bacteriophora
0.9
-
acetylthiocholine pH 8.5, 35°C, isozyme AChEB Heterorhabditis bacteriophora
1.2
-
propionylthiocholine pH 8.5, 35°C, isozyme AChEB Heterorhabditis bacteriophora
1.3
-
acetylthiocholine pH 8.5, 35°C, isozyme AChEA Heterorhabditis bacteriophora
1.8
-
propionylthiocholine pH 8.5, 35°C, isozyme AChEA Heterorhabditis bacteriophora
1.8
-
butyrylthiocholine pH 8.5, 35°C, isozyme AChEB Heterorhabditis bacteriophora
2.8
-
butyrylthiocholine pH 8.5, 35°C, isozyme AChEA Heterorhabditis bacteriophora

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates isozymes AChEA and AChEB Heterorhabditis bacteriophora
Co2+ activates isozymes AChEA and AChEB Heterorhabditis bacteriophora
Mn2+ activates isozymes AChEA and AChEB 4.9 and 6.1fold at 1 mM, respectively Heterorhabditis bacteriophora

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
isozyme isozyme AChEB, gel filtration Heterorhabditis bacteriophora
180000
-
isozyme AChEA, gel filtration Heterorhabditis bacteriophora

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetylcholine + H2O Heterorhabditis bacteriophora
-
choline + acetate
-
?

Organism

Organism UniProt Comment Textmining
Heterorhabditis bacteriophora
-
six different strains, EG1, EG2, EM1, EM2, EM3, and EM4
-

Purification (Commentary)

Purification (Comment) Organism
purification of native isozymes AChEA and AChEB from strain EM2 by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography, to homogeneity Heterorhabditis bacteriophora

Source Tissue

Source Tissue Comment Organism Textmining
additional information the isoenzyme pattern demonstrates two major slow-moving isoforms in all EPN strains examined Heterorhabditis bacteriophora
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
50 220 crude enzyme activity measurement from six different strains, pH 8.5, 35°C Heterorhabditis bacteriophora
1207
-
purified isozyme AChEA, pH 8.5, 35°C Heterorhabditis bacteriophora
1560
-
purified isozyme AChEB, pH 8.5, 35°C Heterorhabditis bacteriophora

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylcholine + H2O
-
Heterorhabditis bacteriophora choline + acetate
-
?
acetylthiocholine + H2O best substrate for both isozymes AChEA and AChEB Heterorhabditis bacteriophora thiocholine + acetate
-
?
benzoylthiocholine + H2O 13% and 9% activity compared to acetylthiocholine with isozymes AChEA and AChEB, respectively Heterorhabditis bacteriophora thiocholine + benzoate
-
?
butyrylthiocholine + H2O 48% and 41% activity compared to acetylthiocholine with isozymes AChEA and AChEB, respectively Heterorhabditis bacteriophora thiocholine + butyrate
-
?
additional information all substrate are used as iodides Heterorhabditis bacteriophora ?
-
?
propionylthiocholine + H2O 71% and 64% activity compared to acetylthiocholine with isozymes AChEA and AChEB, respectively Heterorhabditis bacteriophora thiocholine + propionate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 90000, about, isozyme AChEA, SDS-PAGE Heterorhabditis bacteriophora
monomer 1 * 68000, isozyme AChEB, SDS-PAGE Heterorhabditis bacteriophora

Synonyms

Synonyms Comment Organism
AChE
-
Heterorhabditis bacteriophora
AChEA
-
Heterorhabditis bacteriophora
AChEB
-
Heterorhabditis bacteriophora

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
isozymes AChEA and AChEB Heterorhabditis bacteriophora

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
purified isozymes AChEA and AChEB lose 72% and 65% of their activities upon incubation for 30 min, respectively Heterorhabditis bacteriophora

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
isozymes AChEA and AChEB Heterorhabditis bacteriophora

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.003
-
BW284c51 isozyme isozyme AChEB, pH 8.5, 35°C Heterorhabditis bacteriophora
0.0045
-
BW284c51 isozyme isozyme AChEA, pH 8.5, 35°C Heterorhabditis bacteriophora
0.7
-
eserine isozyme isozyme AChEA, pH 8.5, 35°C Heterorhabditis bacteriophora
0.85
-
eserine isozyme isozyme AChEB, pH 8.5, 35°C Heterorhabditis bacteriophora

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.52
-
isozyme isozyme AChEA, pH 8.5, 35°C Heterorhabditis bacteriophora BW284c51
0.63
-
isozyme isozyme AChEB, pH 8.5, 35°C Heterorhabditis bacteriophora BW284c51
1
-
isozyme isozyme AChEA, pH 8.5, 35°C Heterorhabditis bacteriophora eserine
1.3
-
isozyme isozyme AChEB, pH 8.5, 35°C Heterorhabditis bacteriophora eserine

General Information

General Information Comment Organism
physiological function AChE is the predominant detoxifying enzyme in Heterorhabditis bacteriophora strains that might imply its major role in the detoxification of insecticide(s) Heterorhabditis bacteriophora