Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetylthiocholine | bothe isozymes show substrate inhibition at 10 mM acetylthiocholine | Heterorhabditis bacteriophora | |
BW284c51 | specific and competitive inhibitor, time course of inhibition for the isozymes AChEA and AChEB, inactivation of both within 30 min at 0.6 mM BW284C51 | Heterorhabditis bacteriophora | |
eserine | nonspecific and competitive inhibitor, time course of inhibition for the isozymes AChEA and AChEB, 10% and 30% activity remain after 60 min at 1.0 mM eserine, respectively | Heterorhabditis bacteriophora | |
Hg2+ | AChEA and AChEB are completely inhibited by 1 mM of Hg2+ | Heterorhabditis bacteriophora | |
K+ | AChEA and AChEB lose 80% actiivty at 1 mM of K+ | Heterorhabditis bacteriophora | |
additional information | both isoenzymes are not affected by tetra(monoinopropyl)diphosphotetramide (isoOMPA) at high concentrations up to 50 mM | Heterorhabditis bacteriophora | |
Ni2+ | AChEA and AChEB are completely inhibited by 1 mM of Ni2+ | Heterorhabditis bacteriophora |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics and Lineweaver-Burk plots | Heterorhabditis bacteriophora | |
0.9 | - |
acetylthiocholine | pH 8.5, 35°C, isozyme AChEB | Heterorhabditis bacteriophora | |
1.2 | - |
propionylthiocholine | pH 8.5, 35°C, isozyme AChEB | Heterorhabditis bacteriophora | |
1.3 | - |
acetylthiocholine | pH 8.5, 35°C, isozyme AChEA | Heterorhabditis bacteriophora | |
1.8 | - |
propionylthiocholine | pH 8.5, 35°C, isozyme AChEA | Heterorhabditis bacteriophora | |
1.8 | - |
butyrylthiocholine | pH 8.5, 35°C, isozyme AChEB | Heterorhabditis bacteriophora | |
2.8 | - |
butyrylthiocholine | pH 8.5, 35°C, isozyme AChEA | Heterorhabditis bacteriophora |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates isozymes AChEA and AChEB | Heterorhabditis bacteriophora | |
Co2+ | activates isozymes AChEA and AChEB | Heterorhabditis bacteriophora | |
Mn2+ | activates isozymes AChEA and AChEB 4.9 and 6.1fold at 1 mM, respectively | Heterorhabditis bacteriophora |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
isozyme isozyme AChEB, gel filtration | Heterorhabditis bacteriophora |
180000 | - |
isozyme AChEA, gel filtration | Heterorhabditis bacteriophora |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylcholine + H2O | Heterorhabditis bacteriophora | - |
choline + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Heterorhabditis bacteriophora | - |
six different strains, EG1, EG2, EM1, EM2, EM3, and EM4 | - |
Purification (Comment) | Organism |
---|---|
purification of native isozymes AChEA and AChEB from strain EM2 by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography, to homogeneity | Heterorhabditis bacteriophora |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the isoenzyme pattern demonstrates two major slow-moving isoforms in all EPN strains examined | Heterorhabditis bacteriophora | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
50 | 220 | crude enzyme activity measurement from six different strains, pH 8.5, 35°C | Heterorhabditis bacteriophora |
1207 | - |
purified isozyme AChEA, pH 8.5, 35°C | Heterorhabditis bacteriophora |
1560 | - |
purified isozyme AChEB, pH 8.5, 35°C | Heterorhabditis bacteriophora |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylcholine + H2O | - |
Heterorhabditis bacteriophora | choline + acetate | - |
? | |
acetylthiocholine + H2O | best substrate for both isozymes AChEA and AChEB | Heterorhabditis bacteriophora | thiocholine + acetate | - |
? | |
benzoylthiocholine + H2O | 13% and 9% activity compared to acetylthiocholine with isozymes AChEA and AChEB, respectively | Heterorhabditis bacteriophora | thiocholine + benzoate | - |
? | |
butyrylthiocholine + H2O | 48% and 41% activity compared to acetylthiocholine with isozymes AChEA and AChEB, respectively | Heterorhabditis bacteriophora | thiocholine + butyrate | - |
? | |
additional information | all substrate are used as iodides | Heterorhabditis bacteriophora | ? | - |
? | |
propionylthiocholine + H2O | 71% and 64% activity compared to acetylthiocholine with isozymes AChEA and AChEB, respectively | Heterorhabditis bacteriophora | thiocholine + propionate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 90000, about, isozyme AChEA, SDS-PAGE | Heterorhabditis bacteriophora |
monomer | 1 * 68000, isozyme AChEB, SDS-PAGE | Heterorhabditis bacteriophora |
Synonyms | Comment | Organism |
---|---|---|
AChE | - |
Heterorhabditis bacteriophora |
AChEA | - |
Heterorhabditis bacteriophora |
AChEB | - |
Heterorhabditis bacteriophora |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
isozymes AChEA and AChEB | Heterorhabditis bacteriophora |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
purified isozymes AChEA and AChEB lose 72% and 65% of their activities upon incubation for 30 min, respectively | Heterorhabditis bacteriophora |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
isozymes AChEA and AChEB | Heterorhabditis bacteriophora |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
BW284c51 | isozyme isozyme AChEB, pH 8.5, 35°C | Heterorhabditis bacteriophora | |
0.0045 | - |
BW284c51 | isozyme isozyme AChEA, pH 8.5, 35°C | Heterorhabditis bacteriophora | |
0.7 | - |
eserine | isozyme isozyme AChEA, pH 8.5, 35°C | Heterorhabditis bacteriophora | |
0.85 | - |
eserine | isozyme isozyme AChEB, pH 8.5, 35°C | Heterorhabditis bacteriophora |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.52 | - |
isozyme isozyme AChEA, pH 8.5, 35°C | Heterorhabditis bacteriophora | BW284c51 | |
0.63 | - |
isozyme isozyme AChEB, pH 8.5, 35°C | Heterorhabditis bacteriophora | BW284c51 | |
1 | - |
isozyme isozyme AChEA, pH 8.5, 35°C | Heterorhabditis bacteriophora | eserine | |
1.3 | - |
isozyme isozyme AChEB, pH 8.5, 35°C | Heterorhabditis bacteriophora | eserine |
General Information | Comment | Organism |
---|---|---|
physiological function | AChE is the predominant detoxifying enzyme in Heterorhabditis bacteriophora strains that might imply its major role in the detoxification of insecticide(s) | Heterorhabditis bacteriophora |