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Literature summary for 3.1.1.7 extracted from
- Acetylcholinesterase immobilization and characterization, and comparison of the activity of the porous silicon-immobilized enzyme with its free counterpart (2016), Biosci. Rep., 36, e00311.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | the enzyme immobilized on porous silicon can be exploited as a reusable biocatalyst and for screening of acetylcholinesterase inhibitors from crude plant extracts and synthesized organic compounds | Homo sapiens |
| synthesis | the enzyme immobilized on porous silicon offers a possibilities as a viable biocatalyst in bioprocessing for the chemical and pharmaceutical industries, and bioremediation to enhance productivity and robustness | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | acetylcholinesterase is physically adsorbed on to a mesoporous silicon surface, immobilization method, overview. The porous silicon-immobilized enzyme retains 50% of its activity, shows thermal stability up to 90°C, reusability for up to three cycles, pH stability over a broad range of pH 4.0-9.0, and a shelf-life of 44 days, with an optimal hydrolytic response towards acetylthiocholine iodide at variable drug concentrations | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| neostigmine methyl sulfate | - |
Homo sapiens |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetylcholine + H2O | Homo sapiens | - |
choline + acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P22303 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetylcholine + H2O | - |
Homo sapiens | choline + acetate | - |
? | |
| acetylthiocholine + H2O | substrate is acetylthiocholine iodide, activity detection using 5,5'-dithio-bis(2-nitrobenzoic acid) colorimetric assay and measurement of the absorbance at 412 nm | Homo sapiens | thiocholine + acetate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acetylcholine hydrolase | - |
Homo sapiens |
| AChE | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Homo sapiens |
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