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Literature summary for 3.1.1.7 extracted from

  • Saleem, M.; Rafiq, M.; Seo, S.Y.; Lee, K.H.
    Acetylcholinesterase immobilization and characterization, and comparison of the activity of the porous silicon-immobilized enzyme with its free counterpart (2016), Biosci. Rep., 36, e00311.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
analysis the enzyme immobilized on porous silicon can be exploited as a reusable biocatalyst and for screening of acetylcholinesterase inhibitors from crude plant extracts and synthesized organic compounds Homo sapiens
synthesis the enzyme immobilized on porous silicon offers a possibilities as a viable biocatalyst in bioprocessing for the chemical and pharmaceutical industries, and bioremediation to enhance productivity and robustness Homo sapiens

Engineering

Protein Variants Comment Organism
additional information acetylcholinesterase is physically adsorbed on to a mesoporous silicon surface, immobilization method, overview. The porous silicon-immobilized enzyme retains 50% of its activity, shows thermal stability up to 90°C, reusability for up to three cycles, pH stability over a broad range of pH 4.0-9.0, and a shelf-life of 44 days, with an optimal hydrolytic response towards acetylthiocholine iodide at variable drug concentrations Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
neostigmine methyl sulfate
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetylcholine + H2O Homo sapiens
-
choline + acetate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P22303
-
-

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylcholine + H2O
-
Homo sapiens choline + acetate
-
?
acetylthiocholine + H2O substrate is acetylthiocholine iodide, activity detection using 5,5'-dithio-bis(2-nitrobenzoic acid) colorimetric assay and measurement of the absorbance at 412 nm Homo sapiens thiocholine + acetate
-
?

Synonyms

Synonyms Comment Organism
acetylcholine hydrolase
-
Homo sapiens
AChE
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens