Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.7 extracted from

  • Klichkhanov, N.; Dzhafarova, A.; Al-Rabeei, M.
    Kinetic characteristics of acetylcholinesterase and structural-functional state of rat erythrocyte membranes at moderate hypothermia (2017), Biochemistry (Moscow) Suppl. Ser. A Membr. Cell Biol., 11, 275-286 .
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane integral membrane enzyme Rattus norvegicus 16020
-
synaptic cleft AChE is located on the outer surfaces of postsynaptic membranes of cholinergic synapses in a synaptic cleft Rattus norvegicus 43083
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetylcholine + H2O Rattus norvegicus
-
choline + acetate
-
?
acetylcholine + H2O Rattus norvegicus Wistar
-
choline + acetate
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P37136
-
-
Rattus norvegicus Wistar P37136
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-
endothelial cell
-
Rattus norvegicus
-
erythrocyte
-
Rattus norvegicus
-
hematopoietic cell
-
Rattus norvegicus
-
additional information acetylcholinesterase (AChE) is located on the outer surfaces of postsynaptic membranes of cholinergic synapses in a synaptic cleft. On the other hand, AChE is expressed in non-cholinergic neurons, located in various sites of brain. AChE can be seen not only in neural and muscular tissues, but in a number of non-excitable tissues free of cholinergic innervation, such as testes, endothelial cells, hemopoetic and osteogenic cells, and various tumors. Besides, it can be found at the outer surface of the erythrocyte membrane Rattus norvegicus
-
neuron
-
Rattus norvegicus
-
osteogenic cell
-
Rattus norvegicus
-
testis
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylcholine + H2O
-
Rattus norvegicus choline + acetate
-
?
acetylcholine + H2O
-
Rattus norvegicus Wistar choline + acetate
-
?
acetylthiocholine + H2O activity of AChE is determined by Ellman's method using acetylthiocholine iodide as a substrate Rattus norvegicus thiocholine + acetate
-
?
acetylthiocholine + H2O activity of AChE is determined by Ellman's method using acetylthiocholine iodide as a substrate Rattus norvegicus Wistar thiocholine + acetate
-
?

Synonyms

Synonyms Comment Organism
AChE
-
Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
dependence of the kinetic characteristics of integral membrane enzyme acetylcholinesterase (AChE) and structural and functional state of the membrane on the duration of mild hypothermia, by external cooling to 30°C, microviscosity of erythrocyte membranes is measured, detailed overview. The erythrocyte AChE of a homoiothermic animal is very sensitive to alterations in body temperature. Activity of the enzyme increases essentially immediately after a decrease in body temperature to 30°C and is followed with alterations in its kinetic characteristic Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Rattus norvegicus

General Information

General Information Comment Organism
additional information dependence of the kinetic characteristics of integral membrane enzyme acetylcholinesterase (AChE) and structural and functional state of the membrane on the duration of mild hypothermia, by external cooling to 30°C, microviscosity of erythrocyte membranes is measured, detailed overview. The erythrocyte AChE of a homoiothermic animal is very sensitive to alterations in body temperature. Activity of the enzyme increases essentially immediately after a decrease in body temperature to 30°C and is followed with alterations in its kinetic characteristic Rattus norvegicus
physiological function acetylcholinesterase (AChE) is a key enzyme of cholinergic neurotransmission. The enzyme is located on the outer surfaces of postsynaptic membranes of cholinergic synapses, and its function is regulation of time profiles of neurotransmitter acetylcholine (ACh) concentration in a synaptic cleft. On the other hand, AChE is expressed in non-cholinergic neurons, located in various sites of brain, where no other markers of cholinergic neurotransmission (acetylcholine transferase, high-affinity choline uptake system, etc.) can be found. Therefore, the enzyme has some neuromodulatory functions not involving its catalytic activity. AChE can be seen not only in neural and muscular tissues, but in a number of non-excitable tissues free of cholinergic innervation, such as testes, endothelial cells, hemopoetic and osteogenic cells, and various tumors. Besides, it can be found at the outer surface of the erythrocyte membrane. A rat erythrocyte contains 700-800 molecules of AChE in form of dimers anchored by phosphatidylinositol in an outer membrane layer. A lot of data point to a vital role of AChE in erythrocyte oxygen transport and in the mobilization of nitrogen oxide metabolism in the erythrocytes. Erythrocyte AChE is hardly needed for regulation of the levels of circulating acetylcholine, as the amount of tissue and circulating cholinesterases is more than sufficient to degrade free acetylcholine Rattus norvegicus