KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | integral membrane enzyme | Rattus norvegicus | 16020 | - |
synaptic cleft | AChE is located on the outer surfaces of postsynaptic membranes of cholinergic synapses in a synaptic cleft | Rattus norvegicus | 43083 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylcholine + H2O | Rattus norvegicus | - |
choline + acetate | - |
? | |
acetylcholine + H2O | Rattus norvegicus Wistar | - |
choline + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P37136 | - |
- |
Rattus norvegicus Wistar | P37136 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
endothelial cell | - |
Rattus norvegicus | - |
erythrocyte | - |
Rattus norvegicus | - |
hematopoietic cell | - |
Rattus norvegicus | - |
additional information | acetylcholinesterase (AChE) is located on the outer surfaces of postsynaptic membranes of cholinergic synapses in a synaptic cleft. On the other hand, AChE is expressed in non-cholinergic neurons, located in various sites of brain. AChE can be seen not only in neural and muscular tissues, but in a number of non-excitable tissues free of cholinergic innervation, such as testes, endothelial cells, hemopoetic and osteogenic cells, and various tumors. Besides, it can be found at the outer surface of the erythrocyte membrane | Rattus norvegicus | - |
neuron | - |
Rattus norvegicus | - |
osteogenic cell | - |
Rattus norvegicus | - |
testis | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylcholine + H2O | - |
Rattus norvegicus | choline + acetate | - |
? | |
acetylcholine + H2O | - |
Rattus norvegicus Wistar | choline + acetate | - |
? | |
acetylthiocholine + H2O | activity of AChE is determined by Ellman's method using acetylthiocholine iodide as a substrate | Rattus norvegicus | thiocholine + acetate | - |
? | |
acetylthiocholine + H2O | activity of AChE is determined by Ellman's method using acetylthiocholine iodide as a substrate | Rattus norvegicus Wistar | thiocholine + acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AChE | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
dependence of the kinetic characteristics of integral membrane enzyme acetylcholinesterase (AChE) and structural and functional state of the membrane on the duration of mild hypothermia, by external cooling to 30°C, microviscosity of erythrocyte membranes is measured, detailed overview. The erythrocyte AChE of a homoiothermic animal is very sensitive to alterations in body temperature. Activity of the enzyme increases essentially immediately after a decrease in body temperature to 30°C and is followed with alterations in its kinetic characteristic | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
additional information | dependence of the kinetic characteristics of integral membrane enzyme acetylcholinesterase (AChE) and structural and functional state of the membrane on the duration of mild hypothermia, by external cooling to 30°C, microviscosity of erythrocyte membranes is measured, detailed overview. The erythrocyte AChE of a homoiothermic animal is very sensitive to alterations in body temperature. Activity of the enzyme increases essentially immediately after a decrease in body temperature to 30°C and is followed with alterations in its kinetic characteristic | Rattus norvegicus |
physiological function | acetylcholinesterase (AChE) is a key enzyme of cholinergic neurotransmission. The enzyme is located on the outer surfaces of postsynaptic membranes of cholinergic synapses, and its function is regulation of time profiles of neurotransmitter acetylcholine (ACh) concentration in a synaptic cleft. On the other hand, AChE is expressed in non-cholinergic neurons, located in various sites of brain, where no other markers of cholinergic neurotransmission (acetylcholine transferase, high-affinity choline uptake system, etc.) can be found. Therefore, the enzyme has some neuromodulatory functions not involving its catalytic activity. AChE can be seen not only in neural and muscular tissues, but in a number of non-excitable tissues free of cholinergic innervation, such as testes, endothelial cells, hemopoetic and osteogenic cells, and various tumors. Besides, it can be found at the outer surface of the erythrocyte membrane. A rat erythrocyte contains 700-800 molecules of AChE in form of dimers anchored by phosphatidylinositol in an outer membrane layer. A lot of data point to a vital role of AChE in erythrocyte oxygen transport and in the mobilization of nitrogen oxide metabolism in the erythrocytes. Erythrocyte AChE is hardly needed for regulation of the levels of circulating acetylcholine, as the amount of tissue and circulating cholinesterases is more than sufficient to degrade free acetylcholine | Rattus norvegicus |