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Literature summary for 3.1.1.7 extracted from

  • Xiao, L.S.; Dou, W.; Li, Y.; Wang, J.J.
    Comparative studies of acetylcholinesterase purified from three field populations of Liposcelis entomophila (Enderlein) (Psocoptera: Liposcelididae) (2010), Arch. Insect Biochem. Physiol., 75, 158-173.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Aldicarb
-
Liposcelis entomophila
BW284c51
-
Liposcelis entomophila
omethoate
-
Liposcelis entomophila
propoxur
-
Liposcelis entomophila
serine
-
Liposcelis entomophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.37
-
Acetylcholine enzyme from Sichuan Province population, in 0.1 M phosphate buffer (pH 7.0), at 37°C Liposcelis entomophila
0.48
-
Acetylcholine enzyme from Chongqing Municipality population, in 0.1 M phosphate buffer (pH 7.0), at 37°C Liposcelis entomophila
0.65
-
Acetylcholine enzyme from Hubei Province population, in 0.1 M phosphate buffer (pH 7.0), at 37°C Liposcelis entomophila

Organism

Organism UniProt Comment Textmining
Liposcelis entomophila
-
populations from Sichuan Province, Hubei Province and Chongqing Municipality
-

Purification (Commentary)

Purification (Comment) Organism
procainamide affinity column chromatography Liposcelis entomophila

Source Tissue

Source Tissue Comment Organism Textmining
nymph
-
Liposcelis entomophila
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00149
-
enzyme from Hubei Province population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C Liposcelis entomophila
0.00317
-
enzyme from Sichuan Province population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C Liposcelis entomophila
0.00328
-
enzyme from Chongqing Municipality population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C Liposcelis entomophila
1.18
-
enzyme from Hubei Province population, after 786fold purification, in 0.1 M phosphate buffer (pH 7.0), at 37°C Liposcelis entomophila
1.859
-
enzyme from Sichuan Province population, after 587fold purification, in 0.1 M phosphate buffer (pH 7.0), at 37°C Liposcelis entomophila
2.295
-
enzyme from Chongqing Municipality population, after 700fold purification, in 0.1 M phosphate buffer (pH 7.0), at 37°C Liposcelis entomophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylcholine + H2O
-
Liposcelis entomophila choline + acetate
-
?

Subunits

Subunits Comment Organism
? ? * about 55000, SDS-PAGE Liposcelis entomophila

Synonyms

Synonyms Comment Organism
AChE
-
Liposcelis entomophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37.3
-
optimal temperature for the purified enzyme from Sichuan Province population Liposcelis entomophila
38
-
optimal temperature for the purified enzyme from Hubei Province population Liposcelis entomophila
39.2
-
optimal temperature for the purified enzyme from Chongqing Municipality population Liposcelis entomophila

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
23 25 significant lowest activities are observed at 23°C and 25°C Liposcelis entomophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Liposcelis entomophila

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000011
-
enzyme from Sichuan Province population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C Liposcelis entomophila BW284c51
0.00002
-
enzyme from Chongqing Municipality population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C Liposcelis entomophila BW284c51
0.000049
-
enzyme from Hubei Province population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C Liposcelis entomophila BW284c51
0.000068
-
enzyme from Sichuan Province population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C Liposcelis entomophila propoxur
0.000078
-
enzyme from Chongqing Municipality population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C Liposcelis entomophila propoxur
0.000085
-
enzyme from Hubei Province population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C Liposcelis entomophila propoxur
0.000338
-
enzyme from Sichuan Province population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C Liposcelis entomophila Aldicarb
0.000432
-
enzyme from Chongqing Municipality population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C Liposcelis entomophila Aldicarb
0.000503
-
enzyme from Hubei Province population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C Liposcelis entomophila Aldicarb
0.000823
-
enzyme from Sichuan Province population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C Liposcelis entomophila serine
0.001225
-
enzyme from Chongqing Municipality population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C Liposcelis entomophila serine
0.001263
-
enzyme from Hubei Province population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C Liposcelis entomophila serine
0.01724
-
enzyme from Hubei Province population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C Liposcelis entomophila omethoate
0.02492
-
enzyme from Sichuan Province population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C Liposcelis entomophila omethoate
0.03969
-
enzyme from Chongqing Municipality population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C Liposcelis entomophila omethoate