Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Aldicarb | - |
Liposcelis entomophila | |
BW284c51 | - |
Liposcelis entomophila | |
omethoate | - |
Liposcelis entomophila | |
propoxur | - |
Liposcelis entomophila | |
serine | - |
Liposcelis entomophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.37 | - |
Acetylcholine | enzyme from Sichuan Province population, in 0.1 M phosphate buffer (pH 7.0), at 37°C | Liposcelis entomophila | |
0.48 | - |
Acetylcholine | enzyme from Chongqing Municipality population, in 0.1 M phosphate buffer (pH 7.0), at 37°C | Liposcelis entomophila | |
0.65 | - |
Acetylcholine | enzyme from Hubei Province population, in 0.1 M phosphate buffer (pH 7.0), at 37°C | Liposcelis entomophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Liposcelis entomophila | - |
populations from Sichuan Province, Hubei Province and Chongqing Municipality | - |
Purification (Comment) | Organism |
---|---|
procainamide affinity column chromatography | Liposcelis entomophila |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
nymph | - |
Liposcelis entomophila | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00149 | - |
enzyme from Hubei Province population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C | Liposcelis entomophila |
0.00317 | - |
enzyme from Sichuan Province population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C | Liposcelis entomophila |
0.00328 | - |
enzyme from Chongqing Municipality population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C | Liposcelis entomophila |
1.18 | - |
enzyme from Hubei Province population, after 786fold purification, in 0.1 M phosphate buffer (pH 7.0), at 37°C | Liposcelis entomophila |
1.859 | - |
enzyme from Sichuan Province population, after 587fold purification, in 0.1 M phosphate buffer (pH 7.0), at 37°C | Liposcelis entomophila |
2.295 | - |
enzyme from Chongqing Municipality population, after 700fold purification, in 0.1 M phosphate buffer (pH 7.0), at 37°C | Liposcelis entomophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylcholine + H2O | - |
Liposcelis entomophila | choline + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | ? * about 55000, SDS-PAGE | Liposcelis entomophila |
Synonyms | Comment | Organism |
---|---|---|
AChE | - |
Liposcelis entomophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37.3 | - |
optimal temperature for the purified enzyme from Sichuan Province population | Liposcelis entomophila |
38 | - |
optimal temperature for the purified enzyme from Hubei Province population | Liposcelis entomophila |
39.2 | - |
optimal temperature for the purified enzyme from Chongqing Municipality population | Liposcelis entomophila |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | 25 | significant lowest activities are observed at 23°C and 25°C | Liposcelis entomophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Liposcelis entomophila |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000011 | - |
enzyme from Sichuan Province population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C | Liposcelis entomophila | BW284c51 | |
0.00002 | - |
enzyme from Chongqing Municipality population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C | Liposcelis entomophila | BW284c51 | |
0.000049 | - |
enzyme from Hubei Province population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C | Liposcelis entomophila | BW284c51 | |
0.000068 | - |
enzyme from Sichuan Province population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C | Liposcelis entomophila | propoxur | |
0.000078 | - |
enzyme from Chongqing Municipality population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C | Liposcelis entomophila | propoxur | |
0.000085 | - |
enzyme from Hubei Province population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C | Liposcelis entomophila | propoxur | |
0.000338 | - |
enzyme from Sichuan Province population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C | Liposcelis entomophila | Aldicarb | |
0.000432 | - |
enzyme from Chongqing Municipality population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C | Liposcelis entomophila | Aldicarb | |
0.000503 | - |
enzyme from Hubei Province population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C | Liposcelis entomophila | Aldicarb | |
0.000823 | - |
enzyme from Sichuan Province population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C | Liposcelis entomophila | serine | |
0.001225 | - |
enzyme from Chongqing Municipality population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C | Liposcelis entomophila | serine | |
0.001263 | - |
enzyme from Hubei Province population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C | Liposcelis entomophila | serine | |
0.01724 | - |
enzyme from Hubei Province population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C | Liposcelis entomophila | omethoate | |
0.02492 | - |
enzyme from Sichuan Province population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C | Liposcelis entomophila | omethoate | |
0.03969 | - |
enzyme from Chongqing Municipality population, crude extract, in 0.1 M phosphate buffer (pH 7.0), at 37°C | Liposcelis entomophila | omethoate |