Protein Variants | Comment | Organism |
---|---|---|
additional information | AChE immobilization on two different composite membranes constituted by a chemically modified poly-acrylonitrile membrane plus a layer of tethered chitosan of different molecular weight, 10 kDa or 400 kDa, or directly on a chemically modified poly-acrylonitrile membrane with NaOH and ethylenediamine without chitosan, method development and enzyme activities, the best performance is obtained by the modified poly-acrylonitrile membrane having the chitosan with the lower molecular weight, overview | Electrophorus electricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
paraoxon-methyl | inhibition mechanism and reactivation of paraoxon-methyl-inhibited AChE by pyridine-2-aldoxime methochloride, overview | Electrophorus electricus | |
pyridine-2-aldoxime methochloride | inhibition mechanism | Electrophorus electricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics of soluble and immobilized enzymes, overview | Electrophorus electricus | |
0.9 | - |
acetylthiocholine iodide | pH 7.4, 30°C, soluble free enzyme | Electrophorus electricus | |
2.48 | 9.55 | acetylthiocholine iodide | pH 7.4, 35°C, poly-acrylonitrile-membrane immobilized enzymes | Electrophorus electricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylcholine + H2O | Electrophorus electricus | acetylcholinesterase plays in vivo a key role in cholinergic transmission by catalysing the rapid hydrolysis of the neurotransmitter acetylcholine into acetate and choline | acetate + choline | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Electrophorus electricus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Electrophorus electricus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
AChE activity of the soluble enzyme form and in the insoluble enzyme form with all the three types of modified poly-acrylonitrile membranes, overview | Electrophorus electricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylcholine + H2O | - |
Electrophorus electricus | acetate + choline | - |
? | |
acetylcholine + H2O | acetylcholinesterase plays in vivo a key role in cholinergic transmission by catalysing the rapid hydrolysis of the neurotransmitter acetylcholine into acetate and choline | Electrophorus electricus | acetate + choline | - |
? | |
acetylthiocholine iodide + H2O | detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB | Electrophorus electricus | acetate + thiocholine iodide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AChE | - |
Electrophorus electricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
free enzyme, assay at | Electrophorus electricus |
35 | - |
immobilized enzyme, assay at | Electrophorus electricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Electrophorus electricus |