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Literature summary for 3.1.1.7 extracted from
- Covalent immobilization of Drosophila acetylcholinesterase for biosensor applications (2008), Biotechnol. Appl. Biochem., 52, 257-264.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| AChE expression in Spodoptera frugiperda Sf9 cells using the baculovirus transfection method | Drosophila melanogaster |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | immobilization of AChE on preactivated perlite, a porous silica matrix, by silanization and glutaraldehyde treatment, the immobilization leads to 30% reduced specific activity and improved enzyme stability, e.g. against urea, acetonitrile and temperature, immobilized enzymes retain 80% of its initial activity after 16 consecutive reactor batch cycles, overview | Drosophila melanogaster |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| paraoxon | - |
Drosophila melanogaster |  |
| Urea | the stability of the immobilized recombinant enzyme is 2.7fold increased compared to the soluble recombinant enzyme | Drosophila melanogaster | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| acetonitril | the stability of the immobilized recombinant enzyme is 1.7fold increased compared to the soluble recombinant enzyme | Drosophila melanogaster |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant AChE from Spodoptera frugiperda Sf9 cells | Drosophila melanogaster |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 26240 | - |
purified recombinant immobilized enzyme | Drosophila melanogaster |
| 36370 | - |
purified recombinant enzyme | Drosophila melanogaster |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-acetylthiocholine + H2O | detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB | Drosophila melanogaster | acetate + thiocholine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AChE | - |
Drosophila melanogaster |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
the stability of the immobilized recombinant enzyme is 8.7fold increased compared to the soluble recombinant enzyme | Drosophila melanogaster |
| 60 | - |
the stability of the immobilized recombinant enzyme is 17.7fold increased compared to the soluble recombinant enzyme | Drosophila melanogaster |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Drosophila melanogaster |
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