Crystallization (Comment) | Organism |
---|---|
native enzyme by hanging-drop vapour diffusion method, 4°C, 11.5 mg/ml protein, mother liquor is 32% PEG 200 in 0.15 M MES, pH 5.8-6.2, soaking of crystals at 4°C in mother liquor at pH 6.0, containing either 0.5 mM OTMA for 24 h in 5 ml soaking solution, 20 mM TCh for 12 h in 5 ml soaking solution, 500 mM ATCh for 40 min in 1.5 ml soaking solution, or 20 mM ATCh for 12 h in 200 ml soaking solution, X-ray diffraction structure determination and anaylsis at 2.0-2.7 A resolution | Tetronarce californica |
Protein Variants | Comment | Organism |
---|---|---|
E199D | site-directed mutagenesis, the substrate inhibition is abolished in the mutant enzyme | Tetronarce californica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-oxo-N,N,N-trimethylpentanaminium iodide | i.e. OTMA, a hydrolysable substrate analogue, binding structure analysis | Tetronarce californica | |
Acetylcholine | substrate inhibition due to choline exit being hindered by a substrate molecule bound at the peripheral site | Tetronarce californica | |
acetylthiocholine | interaction with Glu199 of ATCh, substrate inhibition due to choline exit being hindered by a substrate molecule bound at the peripheral site | Tetronarce californica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylcholine + H2O | Tetronarce californica | - |
choline + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tetronarce californica | P04058 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetylcholine + H2O = choline + acetate | reaction mechanism, structure-function relationship analysis, binding structure and mechanism of substrate and product at both the peripheral and active sites, overview | Tetronarce californica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Tetronarce californica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylcholine + H2O | - |
Tetronarce californica | choline + acetate | - |
? | |
acetylcholine + H2O | substrate traffic in AChE involves at least two binding sites, the catalytic and peripheral anionic sites, which are allosterically related and involved in substrate inhibition, structure-function relationship analysis, overview | Tetronarce californica | choline + acetate | - |
? | |
acetylthiocholine iodide + H2O | - |
Tetronarce californica | thiocholine iodide + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | modelling of molecular surfaces of the active-site gorge in the native enzyme, overview | Tetronarce californica |
Synonyms | Comment | Organism |
---|---|---|
AChE | - |
Tetronarce californica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Tetronarce californica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Tetronarce californica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics, data are fitted by multiple nonlinear regressions using a model that assumes two substrate-inhibition mechanisms, overview | Tetronarce californica |