Literature summary for 3.1.1.7 extracted from

Luo, C.; Tong, M.; Chilukuri, N.; Brecht, K.; Maxwell, D.M.; Saxena, A.
An in vitro comparative study on the reactivation of nerve agent-inhibited guinea pig and human acetylcholinesterases by oximes (2007), Biochemistry, 46, 11771-11779.

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Activating Compound

Activating Compound	Comment	Organism	Structure
2-PAM	i.e. 2-[hydroxyiminomethyl]-1-methyl-pyridinium chloride, an oxime that reactivates nerve-agent-inhibited AChE	Cavia porcellus
2-PAM	i.e. 2-[hydroxyiminomethyl]-1-methyl-pyridinium chloride, an oxime that reactivates nerve-agent-inhibited AChE	Homo sapiens
HI-6	i.e. 1-[[[4-(aminocarbonyl)pyridinio]methoxy]methyl]-2-[(hydroxyimino)methyl]-pyridinium dimethanesulfonate, an oxime that reactivates nerve-agent-inhibited AChE	Cavia porcellus
HI-6	i.e. 1-[[[4-(aminocarbonyl)pyridinio]methoxy]methyl]-2-[(hydroxyimino)methyl]-pyridinium dimethanesulfonate, an oxime that reactivates nerve-agent-inhibited AChE	Homo sapiens
HLo-7	i.e. 1-[[[4-(aminocarbonyl)pyridinio]-methoxy]methyl]-2,4-bis-[(hydroxylimino)methyl] pyridinium dimethanesulfonate, an oxime that reactivates nerve-agent-inhibited AChE	Cavia porcellus
HLo-7	i.e. 1-[[[4-(aminocarbonyl)pyridinio]-methoxy]methyl]-2,4-bis-[(hydroxylimino)methyl] pyridinium dimethanesulfonate, an oxime that reactivates nerve-agent-inhibited AChE	Homo sapiens
ICD-585	i.e. 1-(2-hydroxyiminomethyl-1-pyridinium)-1'-(4-carboxy-aminopyridinium)-trimethylene hydrochloride, an oxime that reactivates nerve-agent-inhibited AChE	Cavia porcellus
ICD-585	i.e. 1-(2-hydroxyiminomethyl-1-pyridinium)-1'-(4-carboxy-aminopyridinium)-trimethylene hydrochloride, an oxime that reactivates nerve-agent-inhibited AChE	Homo sapiens
MMB4	i.e. 1,1'-trimethylene bis(4-hydroxyimino methyl) pyridinium dichloride, an oxime that reactivates nerve-agent-inhibited AChE	Cavia porcellus
MMB4	i.e. 1,1'-trimethylene bis(4-hydroxyimino methyl) pyridinium dichloride, an oxime that reactivates nerve-agent-inhibited AChE	Homo sapiens
additional information	the reactivation of nerve agent-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, is the most important step in the treatment of nerve agent poisoning, e.g. by G and V type nerve agents, reactivation kinetics, overview	Cavia porcellus
additional information	the reactivation of nerve agent-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, is the most important step in the treatment of nerve agent poisoning, e.g. by G and V type nerve agents, reactivation kinetics, overview	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
stable expression in CHO cells	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
cyclosarin	reactivation of sarin-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, overview	Cavia porcellus
cyclosarin	reactivation of sarin-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, overview	Homo sapiens
additional information	the reactivation of nerve agent-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, is the most important step in the treatment of nerve agent poisoning, e.g. by G and V type nerve agents, reactivation kinetics, determination of the aging rate constants of nerve agent-inhibited AChE, overview	Cavia porcellus
additional information	the reactivation of nerve agent-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, is the most important step in the treatment of nerve agent poisoning, e.g. by G and V type nerve agents, reactivation kinetics, determination of the aging rate constants of nerve agent-inhibited AChE, overview	Homo sapiens
O-ethyl S-2-N,N-diisopropylaminoethyl methylphosphonothiolate	reactivation of sarin-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, overview	Cavia porcellus
O-ethyl S-2-N,N-diisopropylaminoethyl methylphosphonothiolate	reactivation of sarin-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, overview	Homo sapiens
O-isobutyl S-2-N,N-diethylaminoethyl methylphosphonothiolate	reactivation of sarin-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, overview	Cavia porcellus
O-isobutyl S-2-N,N-diethylaminoethyl methylphosphonothiolate	reactivation of sarin-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, overview	Homo sapiens
sarin	reactivation of sarin-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, overview	Cavia porcellus
sarin	reactivation of sarin-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, overview	Homo sapiens
soman	reactivation of sarin-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, overview	Cavia porcellus
soman	reactivation of sarin-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, overview	Homo sapiens
Tabun	reactivation of sarin-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, overview	Cavia porcellus
Tabun	reactivation of sarin-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, overview	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	anchored to erythrocyte membranes	Cavia porcellus	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Cavia porcellus	the reactivation of nerve agent-inhibited acetylcholinesterase by oxime is the most important step in the treatment of nerve agent poisoning, overview	?	-	?
additional information	Homo sapiens	the reactivation of nerve agent-inhibited acetylcholinesterase by oxime is the most important step in the treatment of nerve agent poisoning, overview	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Cavia porcellus	-	-	-
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme released from erythrocyte membranes anchor by phosphatidylinositol-specific phospholipase C, followed by affinity chromatography on a procainamide resin, and anion exchange chromatography	Cavia porcellus
recombinant enzyme from stably expressing CHO cells by affinity chromatography on a procainamide resin, to homogeneity	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
erythrocyte	-	Cavia porcellus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Cavia porcellus
additional information	-	-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetylthiocholine + H2O	-	Cavia porcellus	thiocholine + acetate	-	?
acetylthiocholine + H2O	-	Homo sapiens	thiocholine + acetate	-	?
additional information	the reactivation of nerve agent-inhibited acetylcholinesterase by oxime is the most important step in the treatment of nerve agent poisoning, overview	Cavia porcellus	?	-	?
additional information	the reactivation of nerve agent-inhibited acetylcholinesterase by oxime is the most important step in the treatment of nerve agent poisoning, overview	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
dimer	-	Cavia porcellus

Synonyms

Synonyms	Comment	Organism
AChE	-	Cavia porcellus
AChE	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Cavia porcellus
25	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Cavia porcellus
8	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)