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Literature summary for 3.1.1.7 extracted from

  • Luo, C.; Tong, M.; Chilukuri, N.; Brecht, K.; Maxwell, D.M.; Saxena, A.
    An in vitro comparative study on the reactivation of nerve agent-inhibited guinea pig and human acetylcholinesterases by oximes (2007), Biochemistry, 46, 11771-11779.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
MMB4 i.e. 1,1'-trimethylene bis(4-hydroxyimino methyl) pyridinium dichloride, an oxime that reactivates nerve-agent-inhibited AChE Cavia porcellus
MMB4 i.e. 1,1'-trimethylene bis(4-hydroxyimino methyl) pyridinium dichloride, an oxime that reactivates nerve-agent-inhibited AChE Homo sapiens
ICD-585 i.e. 1-(2-hydroxyiminomethyl-1-pyridinium)-1'-(4-carboxy-aminopyridinium)-trimethylene hydrochloride, an oxime that reactivates nerve-agent-inhibited AChE Cavia porcellus
ICD-585 i.e. 1-(2-hydroxyiminomethyl-1-pyridinium)-1'-(4-carboxy-aminopyridinium)-trimethylene hydrochloride, an oxime that reactivates nerve-agent-inhibited AChE Homo sapiens
HLo-7 i.e. 1-[[[4-(aminocarbonyl)pyridinio]-methoxy]methyl]-2,4-bis-[(hydroxylimino)methyl] pyridinium dimethanesulfonate, an oxime that reactivates nerve-agent-inhibited AChE Cavia porcellus
HLo-7 i.e. 1-[[[4-(aminocarbonyl)pyridinio]-methoxy]methyl]-2,4-bis-[(hydroxylimino)methyl] pyridinium dimethanesulfonate, an oxime that reactivates nerve-agent-inhibited AChE Homo sapiens
HI-6 i.e. 1-[[[4-(aminocarbonyl)pyridinio]methoxy]methyl]-2-[(hydroxyimino)methyl]-pyridinium dimethanesulfonate, an oxime that reactivates nerve-agent-inhibited AChE Cavia porcellus
HI-6 i.e. 1-[[[4-(aminocarbonyl)pyridinio]methoxy]methyl]-2-[(hydroxyimino)methyl]-pyridinium dimethanesulfonate, an oxime that reactivates nerve-agent-inhibited AChE Homo sapiens
2-PAM i.e. 2-[hydroxyiminomethyl]-1-methyl-pyridinium chloride, an oxime that reactivates nerve-agent-inhibited AChE Cavia porcellus
2-PAM i.e. 2-[hydroxyiminomethyl]-1-methyl-pyridinium chloride, an oxime that reactivates nerve-agent-inhibited AChE Homo sapiens
additional information the reactivation of nerve agent-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, is the most important step in the treatment of nerve agent poisoning, e.g. by G and V type nerve agents, reactivation kinetics, overview Cavia porcellus
additional information the reactivation of nerve agent-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, is the most important step in the treatment of nerve agent poisoning, e.g. by G and V type nerve agents, reactivation kinetics, overview Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
stable expression in CHO cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
cyclosarin reactivation of sarin-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, overview Cavia porcellus
cyclosarin reactivation of sarin-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, overview Homo sapiens
additional information the reactivation of nerve agent-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, is the most important step in the treatment of nerve agent poisoning, e.g. by G and V type nerve agents, reactivation kinetics, determination of the aging rate constants of nerve agent-inhibited AChE, overview Cavia porcellus
additional information the reactivation of nerve agent-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, is the most important step in the treatment of nerve agent poisoning, e.g. by G and V type nerve agents, reactivation kinetics, determination of the aging rate constants of nerve agent-inhibited AChE, overview Homo sapiens
O-ethyl S-2-N,N-diisopropylaminoethyl methylphosphonothiolate reactivation of sarin-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, overview Cavia porcellus
O-ethyl S-2-N,N-diisopropylaminoethyl methylphosphonothiolate reactivation of sarin-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, overview Homo sapiens
O-isobutyl S-2-N,N-diethylaminoethyl methylphosphonothiolate reactivation of sarin-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, overview Cavia porcellus
O-isobutyl S-2-N,N-diethylaminoethyl methylphosphonothiolate reactivation of sarin-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, overview Homo sapiens
sarin reactivation of sarin-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, overview Cavia porcellus
sarin reactivation of sarin-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, overview Homo sapiens
soman reactivation of sarin-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, overview Cavia porcellus
soman reactivation of sarin-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, overview Homo sapiens
Tabun reactivation of sarin-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, overview Cavia porcellus
Tabun reactivation of sarin-inhibited acetylcholinesterase by oximes, 2-PAM, MMB4, HI-6, HLo-7, and ICD-585, overview Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane anchored to erythrocyte membranes Cavia porcellus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Cavia porcellus the reactivation of nerve agent-inhibited acetylcholinesterase by oxime is the most important step in the treatment of nerve agent poisoning, overview ?
-
?
additional information Homo sapiens the reactivation of nerve agent-inhibited acetylcholinesterase by oxime is the most important step in the treatment of nerve agent poisoning, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Cavia porcellus
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme released from erythrocyte membranes anchor by phosphatidylinositol-specific phospholipase C, followed by affinity chromatography on a procainamide resin, and anion exchange chromatography Cavia porcellus
recombinant enzyme from stably expressing CHO cells by affinity chromatography on a procainamide resin, to homogeneity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
Cavia porcellus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Cavia porcellus
additional information
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the reactivation of nerve agent-inhibited acetylcholinesterase by oxime is the most important step in the treatment of nerve agent poisoning, overview Cavia porcellus ?
-
?
additional information the reactivation of nerve agent-inhibited acetylcholinesterase by oxime is the most important step in the treatment of nerve agent poisoning, overview Homo sapiens ?
-
?
acetylthiocholine + H2O
-
Cavia porcellus thiocholine + acetate
-
?
acetylthiocholine + H2O
-
Homo sapiens thiocholine + acetate
-
?

Subunits

Subunits Comment Organism
dimer
-
Cavia porcellus

Synonyms

Synonyms Comment Organism
AChE
-
Cavia porcellus
AChE
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Cavia porcellus
25
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Cavia porcellus
8
-
assay at Homo sapiens