Cloned (Comment) | Organism |
---|---|
cloned from 18-weeks-old fetal cerebral tissue, expression in Pichia pastoris strain GS115, secretion of the active recombinant AChE at high level | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylcholine + H2O | Homo sapiens | key enzyme in the central and peripheral nervous systems where it is responsible for the hydrolysis of neurotransmitter acetylcholine into choline and acetate | choline + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant secreted enzyme from Pichia pastoris strain GS115 culture supernatant by anion-exchange and affinity chromatography to about 96% purity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fetus | fetal cerebral tissue | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
40 | - |
recombinant enzyme in Pichia pastoris culture supernatant | Homo sapiens |
200 | - |
purified recombinant enzyme, activity detection by a simple and specific development reaction with indoxyl acetate as the chromogenic substrate | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylcholine + H2O | - |
Homo sapiens | choline + acetate | - |
? | |
acetylcholine + H2O | key enzyme in the central and peripheral nervous systems where it is responsible for the hydrolysis of neurotransmitter acetylcholine into choline and acetate | Homo sapiens | choline + acetate | - |
? | |
acetylthiocholine + H2O | - |
Homo sapiens | thiocholine + acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AChE | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |