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Literature summary for 3.1.1.7 extracted from

  • Ma, X.; Tan, J.; Wei, D.; Zhu, P.; Sun, M.
    High-level secretion and purification of recombinant acetylcholinesterase from human cerebral tissue in P. pastoris and identification by chromogenic reaction (2006), Appl. Microbiol. Biotechnol., 72, 316-322.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloned from 18-weeks-old fetal cerebral tissue, expression in Pichia pastoris strain GS115, secretion of the active recombinant AChE at high level Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetylcholine + H2O Homo sapiens key enzyme in the central and peripheral nervous systems where it is responsible for the hydrolysis of neurotransmitter acetylcholine into choline and acetate choline + acetate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant secreted enzyme from Pichia pastoris strain GS115 culture supernatant by anion-exchange and affinity chromatography to about 96% purity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
fetus fetal cerebral tissue Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
40
-
recombinant enzyme in Pichia pastoris culture supernatant Homo sapiens
200
-
purified recombinant enzyme, activity detection by a simple and specific development reaction with indoxyl acetate as the chromogenic substrate Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylcholine + H2O
-
Homo sapiens choline + acetate
-
?
acetylcholine + H2O key enzyme in the central and peripheral nervous systems where it is responsible for the hydrolysis of neurotransmitter acetylcholine into choline and acetate Homo sapiens choline + acetate
-
?
acetylthiocholine + H2O
-
Homo sapiens thiocholine + acetate
-
?

Synonyms

Synonyms Comment Organism
AChE
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens