Literature summary for 3.1.1.7 extracted from

Geyer, B.C.; Muralidharan, M.; Cherni, I.; Doran, J.; Fletcher, S.P.; Evron, T.; Soreq, H.; Mor, T.S.
Purification of transgenic plant-derived recombinant human acetylcholinesterase-R (2005), Chem. Biol. Interact., 157-158, 331-334.

Search for more literature for 3.1.1.7

Application

Application	Comment	Organism
synthesis	production of human enzyme by expression in Nicotiana benthamiana with C-terminal SEKDEL endoplasmic retention signal, purified protein is kinetically undistinguishable from commercially available enzyme	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
1,5-bis(allyldimethylammoniumphenyl)pentan-3-one dibromide	-	Homo sapiens
neostigmine	-	Homo sapiens
paraoxon	-	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.15	-	acetylthiocholine	expressed in Nicotiana benthamiana	Homo sapiens
0.21	-	acetylthiocholine	commercial preparation	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	R-isoform, expression in Nicotiana benthamiana, or commercial preparation	-

Purification (Commentary)

Purification (Comment)	Organism
R-isoform, expressed in Nicotiana benthamiana	Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
38	41	-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetylthiocholine + H2O	-	Homo sapiens	thiocholine + acetate	-	?

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Release 2023.1 (January 2023)