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Literature summary for 3.1.1.7 extracted from

  • Kaplan, D.; Barak, D.; Ordentlich, A.; Kronman, C.; Velan, B.; Shafferman, A.
    Is aromaticity essential for trapping the catalytic histidine 447 in human acetylcholinesterase? (2004), Biochemistry, 43, 3129-3136.
    View publication on PubMed
Search for more literature for 3.1.1.7

Protein Variants

Protein Variants Comment Organism
F295A KM-value similar to wild-type Homo sapiens
F295A/F338A strong increase in Km-value, strong decrease in kcat-value Homo sapiens
F295D strong increase in Km-value, strong decrease in kcat-value Homo sapiens
F295D/F338A strong increase in Km-value, strong decrease in kcat-value Homo sapiens
F295I/F338A strong increase in Km-value, strong decrease in kcat-value Homo sapiens
F295L 30% increase in Km-value Homo sapiens
F295L/F338A 2fold increase in Km-value, strong decrease in kcat-value Homo sapiens
F295M KM-value similar to wild-type Homo sapiens
F295M/F338A 70% increase in Km-value, 75% decrease in kcat-value Homo sapiens
F295N/F338A 6fold increase in Km-value, strong decrease in kcat-value Homo sapiens
F295Q/F338A strong increase in Km-value, strong decrease in kcat-value Homo sapiens
F295R strong increase in Km-value, strong decrease in kcat-value Homo sapiens
F295R/F338A 8fold increase in Km-value, strong decrease in kcat-value Homo sapiens
F295S/F338A strong increase in Km-value, strong decrease in kcat-value Homo sapiens
F295T/F338A strong increase in Km-value, strong decrease in kcat-value Homo sapiens
F295V/F338A 12fold increase in Km-value, strong decrease in kcat-value Homo sapiens
F295W/F338A 2fold increase in Km-value, strong decrease in kcat-value Homo sapiens
F295Y 60% decrease in Km-value Homo sapiens
F295Y/F338A 6fold increase in Km-value, strong decrease in kcat-value Homo sapiens
F338A 80% increase in Km-value, 50% decrease in kcat-value Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
diisopropyl phosphorofluoridate
-
 Homo sapiens
soman
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 acetylthiocholine pH 8.0, 27°C, mutant F295Y Homo sapiens
0.13
-
 acetylthiocholine pH 8.0, 27°C, mutant F295A Homo sapiens
0.13
-
 acetylthiocholine pH 8.0, 27°C, mutant F295M Homo sapiens
0.15
-
 acetylthiocholine pH 8.0, 27°C, wild-type Homo sapiens
0.2
-
 acetylthiocholine pH 8.0, 27°C, mutant F295L Homo sapiens
0.22
-
 acetylthiocholine pH 8.0, 27°C, mutant F295M/F338A Homo sapiens
0.24
-
 acetylthiocholine pH 8.0, 27°C, mutant F338A Homo sapiens
0.3
-
 acetylthiocholine pH 8.0, 27°C, mutant F295L/F338A Homo sapiens
0.3
-
 acetylthiocholine pH 8.0, 27°C, mutant F295W/F338A Homo sapiens
0.8
-
 acetylthiocholine pH 8.0, 27°C, mutant F295Y/F338A Homo sapiens
1
-
 acetylthiocholine pH 8.0, 27°C, mutant F295N/F338A Homo sapiens
1.2
-
 acetylthiocholine pH 8.0, 27°C, mutant F295R/F338A Homo sapiens
1.7
-
 acetylthiocholine pH 8.0, 27°C, mutant F295V/F338A Homo sapiens
3
-
 acetylthiocholine pH 8.0, 27°C, mutant F295Q/F338A Homo sapiens
3.4
-
 acetylthiocholine pH 8.0, 27°C, mutant F295R Homo sapiens
4.1
-
 acetylthiocholine pH 8.0, 27°C, mutant F295D Homo sapiens
4.9
-
 acetylthiocholine pH 8.0, 27°C, mutant F295A/F338A Homo sapiens
5
-
 acetylthiocholine pH 8.0, 27°C, mutant F295I/F338A Homo sapiens
5.2
-
 acetylthiocholine pH 8.0, 27°C, mutant F295S/F338A Homo sapiens
5.7
-
 acetylthiocholine pH 8.0, 27°C, mutant F295D/F338A Homo sapiens
14
-
 acetylthiocholine pH 8.0, 27°C, mutant F295T/F338A Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylthiocholine + H2O
-
 Homo sapiens acetate + thiocholine
-
 ?