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Literature summary for 3.1.1.7 extracted from

  • Cohen, O.; Kronman, C.; Chitlaru, T.; Ordentlich, A.; Velan, B.; Shafferman, A.
    Effect of chemical modification of recombinant human acetylcholinesterase by polyethylene glycol on its circulatory longevity (2001), Biochem. J., 357, 795-802.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
C-terminally truncated enzyme Homo sapiens

General Stability

General Stability Organism
circulatory retention time of heterologous AChE in non-human primates is dependent on attachment of polyethylene glycol side chains to lysine residues, retention time is increased by attachment of PEG side chains Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1,5-bis(4-allyldimethylammoniumphenyl)-pentan-3-one dibromide i.e. BW284c51 Homo sapiens
edrophonium
-
Homo sapiens
fasciculin
-
Homo sapiens
propidium
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
acetyl thiocholine pH 8, 27°C, attachment of polyethylene glycol side chains to lysine residues does not alter the Km value Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylthiocholine + H2O
-
Homo sapiens thiocholine + acetate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6500
-
acetyl thiocholine pH 8, 27°C, attachment of polyethylene glycol side chains to lysine residues does not alter the Kcat value Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000008
-
1,5-bis(4-allyldimethylammoniumphenyl)-pentan-3-one dibromide pH 8, 27°C Homo sapiens
0.0008
-
edrophonium pH 8, 27°C Homo sapiens
0.001
-
propidium pH 8, 27°C Homo sapiens
0.8
-
fasciculin pH 8, 27°C Homo sapiens