KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0067 | - |
apigenin 4'-O-beta-D-glucoside | pH 7.5, 30°C | Nemophila menziesii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nemophila menziesii | A0A292GEP7 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Nemophila menziesii | - |
leaf | poor expression | Nemophila menziesii | - |
petal | highly expressed during the early stages of petal development | Nemophila menziesii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | F4'G7GT specifically catalyzes 7-glucosylation of flavone 4'-O-beta-D-glucosides and flavones. No substrates: genistein, daidzein or betanidin. During biosynthesis, apigenin is glucosylated first at the 4'-O-position by 4'-O-glucosyltransferase, and then at the 7-O-position | Nemophila menziesii | ? | - |
- |
|
UDP-alpha-D-glucose + apigenin | reaction of EC 2.4.1.81, 27.8% of the activity with apigenin 4'-O-beta-D-glucoside | Nemophila menziesii | UDP + apigenin 7-O-beta-D-glucoside | - |
? | |
UDP-alpha-D-glucose + apigenin 4'-O-beta-D-glucoside | - |
Nemophila menziesii | UDP + apigenin 4',7-O-beta-D-diglucoside | - |
? | |
UDP-alpha-D-glucose + luteolin | - |
Nemophila menziesii | UDP + luteolin 7-O-beta-D-glucoside | - |
? | |
UDP-alpha-D-glucose + luteolin 4'-O-beta-D-glucoside | 42.4% of the activity with apigenin 4'-O-beta-D-glucoside | Nemophila menziesii | UDP + luteolin 4',7-O-beta-D-diglucoside | - |
? |
Synonyms | Comment | Organism |
---|---|---|
F4'G7GT | - |
Nemophila menziesii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Nemophila menziesii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.73 | - |
apigenin 4'-O-beta-D-glucoside | pH 7.5, 30°C | Nemophila menziesii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Nemophila menziesii |
General Information | Comment | Organism |
---|---|---|
physiological function | Nicotiana tabacum cells expressing both UDP-glucose:flavone 4'-O-glycosyltransferase and F4'G7GT synthesize apigenin 7,4'-O-beta-D-diglucoside in presence of apigenin | Nemophila menziesii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.5 | - |
apigenin 4'-O-beta-D-glucoside | pH 7.5, 30°C | Nemophila menziesii |