Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme exhibits full activity when 10 mM EDTA is present | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
the GST-tagged enzyme is expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
inner membrane | - |
Escherichia coli | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not require the presence of a divalent metal to be enzymatically active | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
2 * 60000, GST-tagged enzyme, SDS-PAGE | Escherichia coli |
61000 | - |
2 * 61000, calculated from amino acid sequence | Escherichia coli |
120000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | Q5J7C6 | - |
- |
Escherichia coli O127:K63(B8) | Q5J7C6 | - |
- |
Purification (Comment) | Organism |
---|---|
glutathione Sepharose column chromatography and Superdex 200 gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-L-fucose + Galbeta(1->3)-GalNAcalpha-OH | - |
Escherichia coli | GDP + Fucalpha(1->2)-Galbeta(1->3)-GalNAcalpha-OH | - |
? | |
GDP-L-fucose + Galbeta(1->3)-GalNAcalpha-OH | - |
Escherichia coli O127:K63(B8) | GDP + Fucalpha(1->2)-Galbeta(1->3)-GalNAcalpha-OH | - |
? | |
GDP-L-fucose + Galbeta(1->3)-GalNAcalpha-OMe | highest activity | Escherichia coli | GDP + Fucalpha(1->2)-Galbeta(1->3)-GalNAcalpha-OMe | - |
? | |
GDP-L-fucose + Galbeta(1->3)-GalNAcalpha-OMe | highest activity | Escherichia coli O127:K63(B8) | GDP + Fucalpha(1->2)-Galbeta(1->3)-GalNAcalpha-OMe | - |
? | |
additional information | the enzyme displays strict substrate specificity, displaying activity only towards acceptors that contain Gal-beta(1->3)-GalNAc-apha-OR linkages and does not recognize lactose, lactulose, Gal-alpha(1->3)-Gal-beta(1->4)Glc, alpha-GalGal-beta(1->4)-glucitol, and beta-D-Gal as acceptors | Escherichia coli | ? | - |
? | |
additional information | the enzyme displays strict substrate specificity, displaying activity only towards acceptors that contain Gal-beta(1->3)-GalNAc-apha-OR linkages and does not recognize lactose, lactulose, Gal-alpha(1->3)-Gal-beta(1->4)Glc, alpha-GalGal-beta(1->4)-glucitol, and beta-D-Gal as acceptors | Escherichia coli O127:K63(B8) | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 60000, GST-tagged enzyme, SDS-PAGE | Escherichia coli |
homodimer | 2 * 61000, calculated from amino acid sequence | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
alpha1->2-fucosyltransferase | - |
Escherichia coli |
alpha1->2-FucT | - |
Escherichia coli |
WbiQ | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | - |
Escherichia coli |