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Literature summary for 2.4.1.65 extracted from

  • Yu, H.; Li, Y.; Wu, Z.; Li, L.; Zeng, J.; Zhao, C.; Wu, Y.; Tasnima, N.; Wang, J.; Liu, H.; Gadi, M.R.; Guan, W.; Wang, P.G.; Chen, X.
    Helicobacter pylori alpha1-3/4-fucosyltransferase (Hp3/4FT)-catalyzed one-pot multienzyme (OPME) synthesis of Lewis antigens and human milk fucosides (2017), Chem. Commun. (Camb.), 53, 11012-11015 .
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
synthesis alpha1-3/4-fucosyltransferase is expressed in Escherichia coli at a level of 30 mg/l culture and used as a diverse catalyst in a one-pot multienzyme system for high-yield production of L-fucose-containing carbohydrates including Lewis antigens such as Lewis a, b, x, O-sulfated Lewis x, and sialyl Lewis x as well as human milk fucosides such as 3-fucosyllactose, lacto-N-fucopentaose III, lacto-N-difuco-hexaose II and III. While difucosylation of tetrasaccharides is readily achieved using an excess amount of donor, synthesis of lacto-N-fucopentaose III is achieved by alpha1-3/4-fucosyltransferase-catalyzed selective fucosylation of the N-acetyllactoamine component in lacto-N-neotetraose Helicobacter pylori

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Helicobacter pylori

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-L-fucose + lacto-N-neotetraose
-
 Helicobacter pylori GDP + ?
-
 ?