Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-Gold (DE3) cells | Glycine max |
expressed in Escherichia coli BL21-Gold (DE3) cells | Acidithiobacillus caldus |
Protein Variants | Comment | Organism |
---|---|---|
L637M/T640V | the double mutant shows a 60fold reduced KM value for UDP and substantially improved UDP-glucose recycling. The mutant is roughly 8times more active than the wild type enzyme | Acidithiobacillus caldus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 13 mM used in assay conditions | Glycine max | |
Mg2+ | 13 mM used in assay conditions | Acidithiobacillus caldus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NDP-alpha-D-glucose + D-fructose | Glycine max | - |
NDP + sucrose | - |
r | |
NDP-alpha-D-glucose + D-fructose | Acidithiobacillus caldus | - |
NDP + sucrose | - |
r | |
NDP-alpha-D-glucose + D-fructose | Acidithiobacillus caldus ATCC 51756 | - |
NDP + sucrose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidithiobacillus caldus | A0A059ZV61 | - |
- |
Acidithiobacillus caldus ATCC 51756 | A0A059ZV61 | - |
- |
Glycine max | P13708 | - |
- |
Purification (Comment) | Organism |
---|---|
His tag affinity chromatography | Acidithiobacillus caldus |
Strep tag affinity chromatography | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NDP-alpha-D-glucose + D-fructose | - |
Glycine max | NDP + sucrose | - |
r | |
NDP-alpha-D-glucose + D-fructose | - |
Acidithiobacillus caldus | NDP + sucrose | - |
r | |
NDP-alpha-D-glucose + D-fructose | - |
Acidithiobacillus caldus ATCC 51756 | NDP + sucrose | - |
r | |
UDP + sucrose | - |
Glycine max | UDP-alpha-D-glucose + D-fructose | - |
r | |
UDP + sucrose | - |
Acidithiobacillus caldus | UDP-alpha-D-glucose + D-fructose | - |
r | |
UDP + sucrose | - |
Acidithiobacillus caldus ATCC 51756 | UDP-alpha-D-glucose + D-fructose | - |
r |
Synonyms | Comment | Organism |
---|---|---|
SuSy | - |
Glycine max |
SuSy | - |
Acidithiobacillus caldus |