Protein Variants | Comment | Organism |
---|---|---|
additional information | PMT1 null mutant shows increased heat lability, alpa-D-mannose transfer only to serine residue of the substrate peptide Ac-YNPTSV-NH2, not to threonine and valine like with the wild-type | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
Ac-Tyr-Ala-Thr-Ala-Val-NH2 | PMT1 | Saccharomyces cerevisiae | |
10 | - |
RSPSPSTQ | additional enzyme form | Saccharomyces cerevisiae | |
15 | - |
Ac-Tyr-Ala-Thr-Ala-Val-NH2 | additional enzyme form | Saccharomyces cerevisiae | |
20 | - |
RSPSPSTQ | PMT1 | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Saccharomyces cerevisiae | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dolichyl phosphate D-mannose + protein | Saccharomyces cerevisiae | - |
dolichyl phosphate + O-D-mannosylprotein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
gene PMT1 | - |
Saccharomyces cerevisiae | - |
enzyme form other than PMT1 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activity of the additional enzyme form is 7fold higher than that of PMT1, substrate Ac-YNPTSV-NH2 | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dolichyl phosphate D-mannose + Ac-Tyr-Ala-Thr-Ala-Val-NH2 | PMT1 transfers preferably to the threonine and valine residues, the additional enzyme form prefers the serine residue, both depending on the sequence of the acceptor substrate peptide | Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosyl-Ac-Tyr-Ala-Thr-Ala-Val-NH2 | - |
? | |
dolichyl phosphate D-mannose + protein | - |
Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosylprotein | - |
? | |
dolichyl phosphate D-mannose + protein | the enzyme transfers mannosyl residues to the hydroxyl of serine or threonine residues | Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosylprotein | - |
? | |
dolichyl phosphate D-mannose + RSPSPSTQ | - |
Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosyl-RSPSPSTQ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PMT | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
additional enzyme form | Saccharomyces cerevisiae |
30 | - |
PMT1 | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
PMT1 null mutant, optimum of the additional enzyme form | Saccharomyces cerevisiae |
7.5 | - |
PMT1 wild-type | Saccharomyces cerevisiae |