Cloned (Comment) | Organism |
---|---|
cloning and overexpression of PMT2 in yeast strain GFUII-4B, showing no alteration of enzyme activity, and co-overexpression with PMT1 in yeast strain TF1.8, leading to 3fold increase in enzyme activity in vitro, thus PMT1 and 2 function as a complex | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Saccharomyces cerevisiae | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
x * 78000, PMT2, SDS-PAGE | Saccharomyces cerevisiae |
92000 | - |
x * 92000, PMT1, SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dolichyl phosphate D-mannose + protein | Saccharomyces cerevisiae | - |
dolichyl phosphate + O-D-mannosylprotein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
gene PMT1 | - |
Saccharomyces cerevisiae | - |
gene PMT2 | - |
Purification (Comment) | Organism |
---|---|
immunoaffinity chromatography, co-purification of PMT1 and PMT2 as a complex, no immuno-cross reactivity | Saccharomyces cerevisiae |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dolichyl phosphate D-mannose + Ac-Tyr-Ala-Thr-Ala-Val-NH2 | recombinant yeast overproducing PMT1 and PMT2 | Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosyl-Ac-Tyr-Ala-Thr-Ala-Val-NH2 | - |
ir | |
dolichyl phosphate D-mannose + Ac-Tyr-Asn-Pro-Thr-Ser-Val-NH2 | - |
Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosyl-Ac-Tyr-Asn-Pro-Thr-Ser-Val-NH2 | - |
ir | |
dolichyl phosphate D-mannose + AcSSSSSNH2 | - |
Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosyl-AcSSSSSNH2 | - |
ir | |
dolichyl phosphate D-mannose + protein | - |
Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosylprotein | - |
? | |
dolichyl phosphate D-mannose + protein | - |
Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosylprotein | - |
ir | |
additional information | PMT1 and PMT2 function as a complex | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 78000, PMT2, SDS-PAGE | Saccharomyces cerevisiae |
? | x * 92000, PMT1, SDS-PAGE | Saccharomyces cerevisiae |
More | PMT1 and PMT2 function as a complex | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
PMT | - |
Saccharomyces cerevisiae |
protein O-mannosyltransferase | - |
Saccharomyces cerevisiae |