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Literature summary for 2.3.2.34 extracted from

  • Huang, D.; Miller, D.; Mathew, R.; Cassell, R.; Holton, J.; Roussel, M.; Schulman, B.
    A unique E1-E2 interaction required for optimal conjugation of the ubiquitin-like protein NEDD8 (2004), Nat. Struct. Mol. Biol., 11, 927-935 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.2.34

Protein Variants

Protein Variants Comment Organism
F5A the mutant shows a strongly decreased Km value for E1 NEDD8-activating enzyme compared to the wild type Homo sapiens
F5A/L7A the mutant shows a strongly decreased Km value for E1 NEDD8-activating enzyme compared to the wild type Homo sapiens
K11A the mutant shows a wild type Km value for E1 NEDD8-activating enzyme Homo sapiens
K12A the mutant shows a decreased Km value for E1 NEDD8-activating enzyme compared to the wild type Homo sapiens
L4A the mutant shows a decreased Km value for E1 NEDD8-activating enzyme compared to the wild type Homo sapiens
L4A/F5A/L7A the mutant shows a strongly decreased Km value for E1 NEDD8-activating enzyme compared to the wild type Homo sapiens
L7A the mutant shows a strongly decreased Km value for E1 NEDD8-activating enzyme compared to the wild type Homo sapiens
M1A the mutant shows an increased Km value for E1 NEDD8-activating enzyme compared to the wild type Homo sapiens
Q10A the mutant shows a wild type Km value for E1 NEDD8-activating enzyme Homo sapiens
Q9A the mutant shows a decreased Km value for E1 NEDD8-activating enzyme compared to the wild type Homo sapiens
S6A the mutant shows a decreased Km value for E1 NEDD8-activating enzyme compared to the wild type Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine + [E2 NEDD8-conjugating enzyme]-L-cysteine Homo sapiens
-
 [E1 NEDD8-activating enzyme]-L-cysteine + [E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-yl-L-cysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P61081
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine + [E2 NEDD8-conjugating enzyme]-L-cysteine
-
 Homo sapiens [E1 NEDD8-activating enzyme]-L-cysteine + [E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-yl-L-cysteine
-
 ?

Synonyms

Synonyms Comment Organism
NEDD8 E2
-
 Homo sapiens
Ubc12
-
 Homo sapiens

General Information

General Information Comment Organism
metabolism the N-terminal extension of Ubc12 is a selective peptide motif that optimally recruits NEDD8 E1 for the NEDD8 transfer cascade and plays an important role in mitogenesis Homo sapiens