Literature summary for 2.3.2.34 extracted from

Monda, J.; Scott, D.; Miller, D.; Lydeard, J.; King, D.; Harper, J.; Bennett, E.; Schulman, B.
Structural conservation of distinctive N-terminal acetylation-dependent interactions across a family of mammalian NEDD8 ligation enzymes (2013), Structure, 21, 42-53 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in NIH-3T3 cells and Sf9 insect cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 2.28 M sodium malonate, pH 7.0	Homo sapiens
hanging drop vapor diffusion method, using 20% (w/v) PEG2000 MME, 0.1 M NaBr, 3% (w/v) sorbitol	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
[E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine + [E2 NEDD8-conjugating enzyme]-L-cysteine	Homo sapiens	-	[E1 NEDD8-activating enzyme]-L-cysteine + [E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-yl-L-cysteine	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P61081	-	-
Homo sapiens	Q969M7	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	RBX2 is specific for UBE2F, which mediates neddylation of the RBX2 partner, cullin 5	Homo sapiens	?	-	-
additional information	UBC12 is specific for RBX1 and mediates neddylation of RBX1-associated cullins 1, 2, 3 and 4	Homo sapiens	?	-	-
[E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine + [E2 NEDD8-conjugating enzyme]-L-cysteine	-	Homo sapiens	[E1 NEDD8-activating enzyme]-L-cysteine + [E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-yl-L-cysteine	-	?

Synonyms

Synonyms	Comment	Organism
NEDD8 E2	-	Homo sapiens
Ubc12	-	Homo sapiens
UBE2F	-	Homo sapiens

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Release 2023.1 (January 2023)