Protein Variants | Comment | Organism |
---|---|---|
T399A | complete loss of enzymatic activity | Bacillus licheniformis |
T399C | complete loss of enzymatic activity | Bacillus licheniformis |
T399S | dramatic reduction in activity | Bacillus licheniformis |
T399S | precursor is able to precede a time-dependent autocatalytic process to generate the 44.9 and 21.7 kDa subunits of mature enzyme | Bacillus licheniformis |
T417A | complete loss of enzymatic activity | Bacillus licheniformis |
T417A | precursor is able to precede a time-dependent autocatalytic process to generate the 44.9 and 21.7 kDa subunits of mature enzyme, processed enzyme lacks activity | Bacillus licheniformis |
T417K | complete loss of enzymatic activity | Bacillus licheniformis |
T417S | dramatic reduction in activity | Bacillus licheniformis |
T417S | precursor is able to precede a time-dependent autocatalytic process to generate the 44.9 and 21.7 kDa subunits of mature enzyme | Bacillus licheniformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
5-L-glutamyl-4-nitroanilide | wild-type, pH 8.0, 40°C | Bacillus licheniformis | |
0.09 | - |
5-L-glutamyl-4-nitroanilide | mutant T417S, pH 8.0, 40°C | Bacillus licheniformis | |
0.11 | - |
5-L-glutamyl-4-nitroanilide | mutant T399S, pH 8.0, 40°C | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.25 | - |
mutant T399S, pH 8.0, 40°C | Bacillus licheniformis |
12.7 | - |
mutant T417S, pH 8.0, 40°C | Bacillus licheniformis |
83.7 | - |
wild-type, pH 8.0, 40°C | Bacillus licheniformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-L-glutamyl-4-nitroanilide + Gly-Gly | - |
Bacillus licheniformis | 4-nitroaniline + 5-L-glutamyl-Gly-Gly | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.42 | - |
5-L-glutamyl-4-nitroanilide | mutant T399S, pH 8.0, 40°C | Bacillus licheniformis | |
24.24 | - |
5-L-glutamyl-4-nitroanilide | mutant T417S, pH 8.0, 40°C | Bacillus licheniformis | |
109.5 | - |
5-L-glutamyl-4-nitroanilide | wild-type, pH 8.0, 40°C | Bacillus licheniformis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13 | - |
5-L-glutamyl-4-nitroanilide | mutant T399S, pH 8.0, 40°C | Bacillus licheniformis | |
269.5 | - |
5-L-glutamyl-4-nitroanilide | mutant T417S, pH 8.0, 40°C | Bacillus licheniformis | |
1504 | - |
5-L-glutamyl-4-nitroanilide | wild-type, pH 8.0, 40°C | Bacillus licheniformis |