KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
puromycin | at 37°C and pH 7.5 | Escherichia coli | |
3.2 | - |
cytidine-puromycin | at 37°C and pH 7.5 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the velocity of the reaction measured at limiting concentrations of Met-tRNA and saturating puromycin increases with Mg2+ concentration up to about 100 mM | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Met-tRNA + cytidine-puromycin | - |
Escherichia coli | tRNA + Met-cytidine-puromycin | - |
? | |
Met-tRNA + puromycin | - |
Escherichia coli | tRNA + Met-puromycin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
peptidyl transferase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
puromycin | at 37°C and pH 7.5 | Escherichia coli | |
19 | - |
cytidine-puromycin | at 37°C and pH 7.5 | Escherichia coli |