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Literature summary for 2.3.1.B44 extracted from

  • Goris, M.; Magin, R.S.; Foyn, H.; Myklebust, L.M.; Varland, S.; Ree, R.; Drazic, A.; Bhambra, P.; Stoeve, S.I.; Baumann, M.; Haug, B.E.; Marmorstein, R.; Arnesen, T.
    Structural determinants and cellular environment define processed actin as the sole substrate of the N-terminal acetyltransferase NAA80 (2018), Proc. Natl. Acad. Sci. USA, 115, 4405-4410 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.B44

Crystallization (Commentary)

Crystallization (Comment) Organism
structure of NAA80 in complex with an inhibitor. NAA80 adopts a fold similar to other N-acetyltransferases but with a more open substrate binding region Homo sapiens

Protein Variants

Protein Variants Comment Organism
K73A mutation corresponding to residue that interacts with D3 of substrate, reduction in activity Homo sapiens
R43A mutation corresponding to residue that interacts with D2 of substrate, loss of activity Homo sapiens
S88A mutation corresponding to residue that interacts with D3 of substrate, reduction of activity Homo sapiens
W36A mutation corresponding to residue that interacts with D2 of substrate, loss of activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
coenzyme A-acetyl-DDDI-NH2
-
 Homo sapiens
coenzyme A-acetyl-EEEI-NH2
-
 Homo sapiens
coenzyme A-acetyl-MDEL-NH2
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0424
-
 EEEI24 pH not specified in the publication, temperature not specified in the publication Homo sapiens
0.0505
-
 DDDI24 pH not specified in the publication, temperature not specified in the publication Homo sapiens
0.119
-
 MDEL24 pH not specified in the publication, temperature not specified in the publication Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q93015
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + DDDI24
-
 Homo sapiens CoA + N-acetyl-DDDI24
-
 ?
acetyl-CoA + EEEI24
-
 Homo sapiens CoA + N-acetyl-EEEI24
-
 ?
acetyl-CoA + MDDD24
-
 Homo sapiens CoA + N-acetyl-MDDD24
-
 ?
acetyl-CoA + MDEL24
-
 Homo sapiens CoA + N-acetyl-MDEL24
-
 ?
additional information best substrate for NAA80 is MDEL24, with a fivefold higher product formation compared with the second best substrate, MDDD24, while its cellular substrate, processed beta-actin, DDDI24 ranks third. The substrate specificity of NAA80 is mainly derived through interactions between the enzyme and the acidic amino acids at positions 2 and 3 of the actin substrate and not residues 1 and 2. NAA80 uses a ternary complex mechanism. No substrate: MELL24 Homo sapiens ?
-
-

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000043
-
 coenzyme A-acetyl-DDDI-NH2 pH not specified in the publication, temperature not specified in the publication Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00038
-
 pH not specified in the publication, temperature not specified in the publication Homo sapiens coenzyme A-acetyl-DDDI-NH2

General Information

General Information Comment Organism
physiological function ectopic expression of NAA80 in a yeast strain lacking NatB activity partially restores N-terminal acetylation of NatB substrates, including yeast actin Homo sapiens