Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activation | Juglans sp. | |
cysteine | dependent on | Juglans sp. | |
Dowex | 77% increase of activity | Juglans sp. | |
EDTA | 50 mM, 9% increase of activity | Juglans sp. | |
N2 | 79% increase of activity | Juglans sp. | |
Polyethyleneglycol | activity strongly dependent on 1.5% | Juglans sp. | |
Polyvinylpyrrolidone | 10% w/v essential | Juglans sp. | |
sucrose | 25% increase of activity | Juglans sp. |
General Stability | Organism |
---|---|
0.1% bovine serum albumin stabilizes | Juglans sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-coumaroyl-CoA | substrate inhibition above 0.03 mM | Juglans sp. | |
malonyl-CoA | - |
Juglans sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
4-coumaroyl-CoA | - |
Juglans sp. | |
0.0026 | - |
malonyl-CoA | - |
Juglans sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 1 mM, 15% increase of activity | Juglans sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Juglans sp. | - |
walnut, Juglans nigra * Juglans regia | - |
Oxidation Stability | Organism |
---|---|
sensitive to oxygen | Juglans sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bark | - |
Juglans sp. | - |
Storage Stability | Organism |
---|---|
-70°C, 0.1% bovine serum albumin, at least 3 weeks | Juglans sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + 4-coumaroyl-CoA | - |
Juglans sp. | naringenin chalcone + CoA + CO2 | due to the presence of isomerase the immediate product naringenin chalcone is not detectable, instead 4,2',4',6'-tetrahydroxychalcone is found | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Juglans sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | less than 40% of maximal activity above and below | Juglans sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Juglans sp. |
8 | - |
4-coumaroyl-CoA | Juglans sp. |