Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Sphingobacterium multivorum |
expressed in Escherichia coli | Sphingobacterium spiritivorum |
expressed in Escherichia coli | Bacteriovorax stolpii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
palmitoyl CoA | enzyme shows remarkable substrate inhibition at palmitoyl-CoA concentrations higher than 0.1 nM | Bacteriovorax stolpii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
palmitoyl-CoA | - |
Sphingobacterium multivorum | |
0.39 | - |
palmitoyl-CoA | - |
Sphingobacterium spiritivorum | |
3.7 | - |
L-serine | - |
Bacteriovorax stolpii | |
4.8 | - |
L-serine | - |
Sphingobacterium multivorum | |
5 | - |
L-serine | - |
Sphingobacterium spiritivorum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peripheral membrane protein | by immunoelectron microscopy it is shown that the protein is bound to the inner membrane of cells as peripheral membrane protein | Sphingobacterium multivorum | - |
- |
peripheral membrane protein | by immunoelectron microscopy it is shown that the protein is bound to the inner membrane of cells as peripheral membrane protein | Sphingobacterium spiritivorum | - |
- |
peripheral membrane protein | by immunoelectron microscopy it is shown that the protein is bound to the inner membrane of cells as peripheral membrane protein | Bacteriovorax stolpii | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
SDS-PAGE | Sphingobacterium multivorum |
45000 | - |
SDS-PAGE | Sphingobacterium spiritivorum |
50000 | - |
SDS-PAGE | Bacteriovorax stolpii |
90000 | - |
gel filtration | Sphingobacterium multivorum |
90000 | - |
gel filtration | Sphingobacterium spiritivorum |
90000 | - |
gel filtration | Bacteriovorax stolpii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteriovorax stolpii | A7BFV8 | - |
- |
Sphingobacterium multivorum | A7BFV6 | - |
- |
Sphingobacterium spiritivorum | A7BFV7 | - |
- |
Purification (Comment) | Organism |
---|---|
purified as a water-soluble active homodimer | Sphingobacterium spiritivorum |
purified as a water-soluble active homodimer | Bacteriovorax stolpii |
purified as water-soluble active homodimers | Sphingobacterium multivorum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + L-serine | - |
Sphingobacterium multivorum | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
palmitoyl-CoA + L-serine | - |
Sphingobacterium spiritivorum | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
palmitoyl-CoA + L-serine | - |
Bacteriovorax stolpii | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | gel filtration | Sphingobacterium multivorum |
homodimer | gel filtration | Sphingobacterium spiritivorum |
homodimer | gel filtration | Bacteriovorax stolpii |
Synonyms | Comment | Organism |
---|---|---|
serine palmitoyltransferase | - |
Sphingobacterium multivorum |
serine palmitoyltransferase | - |
Sphingobacterium spiritivorum |
serine palmitoyltransferase | - |
Bacteriovorax stolpii |
SPT | - |
Sphingobacterium multivorum |
SPT | - |
Sphingobacterium spiritivorum |
SPT | - |
Bacteriovorax stolpii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
palmitoyl-CoA | - |
Bacteriovorax stolpii | |
0.12 | - |
palmitoyl-CoA | - |
Sphingobacterium multivorum | |
0.15 | - |
palmitoyl-CoA | - |
Sphingobacterium spiritivorum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Sphingobacterium spiritivorum |
7 | 8 | - |
Bacteriovorax stolpii |
7.4 | 8 | - |
Sphingobacterium multivorum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
protein denatures below pH 7.2 | Sphingobacterium multivorum |
6.8 | 8.5 | 90% activity | Sphingobacterium spiritivorum |
6.8 | 8.5 | 90% activity | Bacteriovorax stolpii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Sphingobacterium multivorum | |
pyridoxal 5'-phosphate | - |
Sphingobacterium spiritivorum | |
pyridoxal 5'-phosphate | - |
Bacteriovorax stolpii |