Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lipid droplet | - |
Saccharomyces cerevisiae | 5811 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharomyces cerevisiae | NuA4 targets histone and nonhistone proteins | ? | - |
- |
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additional information | Saccharomyces cerevisiae BY4741 | NuA4 targets histone and nonhistone proteins | ? | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q08649 | NuA4 is a 13-subunit KAT complex containing the essential catalytic domain Esa1 | - |
Saccharomyces cerevisiae BY4741 | Q08649 | NuA4 is a 13-subunit KAT complex containing the essential catalytic domain Esa1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | NuA4 targets histone and nonhistone proteins | Saccharomyces cerevisiae | ? | - |
- |
|
additional information | NuA4 targets histone and nonhistone proteins | Saccharomyces cerevisiae BY4741 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
multimer | the NuA4 histone acetyltransferase complex is composed of at least ACT1, ARP4, EAF3, EAF5, EAF6, EAF7, EPL1, ESA1, SWC4, TRA1, VID21, YAF9 and YNG2 subunits | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
lysine acetyltransferase complex | - |
Saccharomyces cerevisiae |
NuA4 | the NuA4 histone acetyltransferase complex is composed of at least ACT1, ARP4, EAF3, EAF5, EAF6, EAF7, EPL1, ESA1, SWC4, TRA1, VID21, YAF9 and YNG2 subunits | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | NuA4 mutants induce the expression of the inositol-3-phosphate synthase gene, INO1, which leads to excessive accumulation of inositol, a key metabolite used for phospholipid biosynthesis, called an Opi- phenotype. High-throughput genomic screens have identified many other mutants that derepress INO1 transcription, besides Opil mutants, and cause excessive accumulation of inositol, including mutants of the NuA4 complex (EAF1, EAF3, EAF5, EAF7, YAF9, and ESA1). NuA4 mutants exacerbate the growth defects of sec14-1ts under inositol-depleted conditions. As NuA4 mutants exhibit a derepression of INO1 and excessive inositol production, or Opi- phenotype, NuA4 mutants suppress the growth defect in sec14-1ts under inositol-depleted conditions. Lipid droplet dynamics are impaired in eaf1DELTA cells. The eaf1DELTA mutant negative genetic interaction with sec14-1ts and the decreased lipid droplet staining in eaf1D originate from defects within the fatty acid biosynthesis pathway | Saccharomyces cerevisiae |
metabolism | analysis of connections between NuA4, inositol, and Sec14, which is a phosphatidylinositol/phosphatidylcholine transfer protein. Overview of phospholipid metabolism. Sec14 (UniProt ID P24280) is an essential phospholipid-binding protein that coordinates the metabolism of phosphatidylinositol-4-phosphate with phosphatidylcholine (PC) at the Golgi to create a lipid environment necessary for trafficking events | Saccharomyces cerevisiae |
additional information | NuA4 is a 13-subunit KAT complex containing the essential catalytic domain Esa1 and held together by the scaffolding protein Eaf1 | Saccharomyces cerevisiae |
physiological function | the lysine acetyltransferase complex NuA4 plays a role in phospholipid homeostasis. One role for NuA4 is the regulation of chromatin remodeling and gene transcription through the acetylation of histones H4 andH2A-Z, and NuA4 also targets nonhistone proteins | Saccharomyces cerevisiae |