Cloned (Comment) | Organism |
---|---|
recombinant expression of the inducible HA-tagged wild-type enzyme and dominant-negative mutant E703G in Toxoplasma gondii parasites | Toxoplasma gondii |
Protein Variants | Comment | Organism |
---|---|---|
E703G | the point mutation ablates enzymatic activity, dominant-negative form of GCN5b. Replication arrest observed in ddHAGCN5b(E703G) parasites is due to a reduction in histone acetylation, which in turn leads to dysregulation of the transcriptome | Toxoplasma gondii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + histone H3 | Toxoplasma gondii | - |
CoA + acetylhistone H3 | - |
? | |
additional information | Toxoplasma gondii | enzyme GCN5b interacts with AP2-domain proteins, apicomplexan plant-like transcription factors, as well as a core complex that includes the co-activator ADA2-A, TFIID subunits, LEO1 polymerase-associated factor (Paf1) subunit, and RRM proteins | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Toxoplasma gondii | S8F3V2 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
tachyzoite | - |
Toxoplasma gondii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + histone H3 | - |
Toxoplasma gondii | CoA + acetylhistone H3 | - |
? | |
additional information | enzyme GCN5b interacts with AP2-domain proteins, apicomplexan plant-like transcription factors, as well as a core complex that includes the co-activator ADA2-A, TFIID subunits, LEO1 polymerase-associated factor (Paf1) subunit, and RRM proteins | Toxoplasma gondii | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GCN5b | - |
Toxoplasma gondii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Toxoplasma gondii |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the GCN5-family of lysine acetyltransferases | Toxoplasma gondii |
malfunction | induced accumulation of the ddHAGCN5b(E703G) protein leads to a rapid arrest in parasite replication. Growth arrest is accompanied by a decrease in histone H3 acetylation at specific lysine residues as well as reduced expression of GCN5b target genes in GCN5b(E703G) parasites, which are identified using chromatin immunoprecipitation coupled with microarray hybridization | Toxoplasma gondii |
additional information | mechanism by which GCN5b is recruited to target genes by co-purifying the enzyme with plant-like AP2-domain proteins, a subset of which function as DNA-binding transcription factors in Apicomplexa, overview | Toxoplasma gondii |
physiological function | enzyme GCN5b seems to be essential for Toxoplasma viability. GCN5b plays a central role in transcriptional and chromatin remodeling complexes. GCN5b has a non-redundant and indispensable role in regulating gene expression required during the Toxoplasma lytic cycle GCN5b is an essential driver of tachyzoite proliferation | Toxoplasma gondii |