Cloned (Comment) | Organism |
---|---|
expression in sat1 sat2 mutant cells of Saccharomyces cerevisiae | Homo sapiens |
two genes, SAT1 and SAT2 encode isozymes of the enzyme | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CI-976 | cell-free extract of sat1 and sat2 cells: more than 95% inhibition at 0.2 mM, intact sat1 and sat2 cells: approximately 75-80% inhibition at 0.2 mM | Homo sapiens | |
CI-976 | cell-free extract of wild-type cells: approximately 90% inhibition at 0.1 mM and more than 95% inhibition at 0.2 mM, intact wild-type cells: approximately 75-80% inhibition at 0.2 mM | Saccharomyces cerevisiae | |
DuP 128 | - |
Homo sapiens | |
DuP 128 | approximately 50% inhibition at 0.03-0.04 mM | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
recombinant enzyme | Homo sapiens |
71600 | - |
deduced from amino acid sequence data | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Saccharomyces cerevisiae | P53629 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | there are six potential N-linked glycosylation sites in sat1, two of which are conserved in sat2 | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
when yeast-expressed enzyme activity is measured in the presence of ergosterol liposomes, specific activity is reduced more than 80%, recombinant enzyme is active but unable to esterify ergosterol efficiently | Homo sapiens |
0.0009 | - |
recombinant enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oleoyl-CoA + cholesterol | - |
Saccharomyces cerevisiae | CoA + cholesteryl oleate | - |
? | |
oleoyl-CoA + cholesterol | recombinant enzyme | Homo sapiens | CoA + cholesteryl oleate | - |
? | |
oleoyl-CoA + ergosterol | endogenous ergosterol as substrate or exogenous ergosterol in the form of ergosterol/phosphatidylcholine vesicles as substrate | Saccharomyces cerevisiae | CoA + ergosteryl oleate | - |
? | |
oleoyl-CoA + ergosterol | recombinant enzyme: restores ergosteryl oleate formation in vivo to only approximately 8% of that catalysed by yeast enzyme in wild-type cells | Homo sapiens | CoA + ergosteryl oleate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Saccharomyces cerevisiae |