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Literature summary for 2.3.1.255 extracted from

  • Chien, M.H.; Lee, W.J.; Yang, Y.C.; Tan, P.; Pan, K.F.; Liu, Y.C.; Tsai, H.C.; Hsu, C.H.; Wen, Y.C.; Hsiao, M.; Hua, K.T.
    N-alpha-acetyltransferase 10 protein promotes metastasis by stabilizing matrix metalloproteinase-2 protein in human osteosarcomas (2018), Cancer Lett., 433, 86-98 .
    View publication on PubMed
Search for more literature for 2.3.1.255

Cloned(Commentary)

Cloned (Comment) Organism
gene NAA10, recombinant expression of the enzyme in human 293T cells using a lentiviral vector, recombinant expression of GST-tagged wild-type and mutant Naa10 enzymes in Escherichia coli strain BL21 Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information knockdown of Naa10p by shRNAs, knockdown efficiencies, overview. Generation of truncated Naa10p mutants Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P41227
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged wild-type and mutant Naa10 enzymes from Escherichia coli strain BL21 by glutathione affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
143-B cell
-
 Homo sapiens
-
bone
-
 Homo sapiens
-
HOS cell
-
 Homo sapiens
-
MG-63 cell
-
 Homo sapiens
-
MNNG/HOS cell
-
 Homo sapiens
-
additional information enzyme levels in different osteosarcoma cell lines, overview Homo sapiens
-
osteoblast located around new bone surfaces Homo sapiens
-
osteosarcoma cell higher NAA10 transcripts in metastatic osteosarcoma tissues compared to non-metastatic tissues. MMP-2 expression levels are also significantly correlated with Naa10p levels in osteosarcoma tissues Homo sapiens
-
SAOS-2 cell
-
 Homo sapiens
-
U2-OS cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + N-terminal L-methionyl-[MMP2] matrix metalloproteinase-2, MMP2, with sequence MEALMAR Homo sapiens CoA + H+ + N-terminal Nalpha-acetyl-L-methionyl-[MMP2]
-
 ?
additional information no activity with an MMP2 mutated at the acetylytion site of Naa10 Homo sapiens ?
-
-

Synonyms

Synonyms Comment Organism
N-alpha-acetyltransferase 10
-
 Homo sapiens
NAA10
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
 Homo sapiens

General Information

General Information Comment Organism
malfunction knockdown and overexpression of Naa10p in osteosarcoma cells respectively leads to decreased and increased cell migratory/invasive abilities. Re-expression of Naa10p, but not of an enzymatically inactive mutant, relieves suppression of the invasive ability in vitro and metastasis in vivo imposed by Naa10p-knockdown. The matrix metalloproteinase (MMP)-2 is responsible for the Naa10p-induced invasive phenotype Homo sapiens
physiological function N-alpha-acetyltransferase 10 protein (Naa10p) mediates N-terminal acetylation of nascent proteins. It promotes metastasis by stabilizing matrix metalloproteinase-2 protein in human osteosarcomas via its N-terminal acetylation activity. Oncogenic role of Naa10p, overview. Higher NAA10 transcripts are observed in metastatic osteosarcoma tissues compared to non-metastatic tissues and are also correlated with a worse prognosis of patients. Naa10p is directly associated with MMP-2 protein through its acetyltransferase domain and maintains MMP-2 protein stability via NatA complex activity. MMP-2 expression levels are also significantly correlated with Naa10p levels in osteosarcoma tissues. Function of Naa10p in the regulation of cell invasiveness by preventing MMP-2 protein degradation that is crucial during osteosarcoma metastasis. Naa10p promotes migratory/invasive abilities of osteosarcoma cells, it regulates cell invasion of the osteosarcoma cell lines Homo sapiens