Cloned (Comment) | Organism |
---|---|
overexpressed both as native and as fusion protein with a His6 tag at the C- or the N-terminus in human embryonic kidney-293 cells expressing the large T-antigen of simian virus 40 (HEK-293T cells) | Homo sapiens |
overexpressed both as native and as fusion proteins with a His6 tag at the C- or the N-terminus in human embryonic kidney-293 cells expressing the large T-antigen of simian virus 40 (HEK-293T cells); furthermore expressed as fusion protein in rat embryonal cortical neurons and in Chinese-hamster ovary cells | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glutamate | Glutamate is a competitive inhibitor. No inhibition is observed with other amino acids, indicating that the enzyme is specific. | Mus musculus | |
N-acetyl-L-aspartate | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
acetyl-CoA | in presence of 0.5 mM CHAPS | Mus musculus | |
0.013 | - |
acetyl-CoA | in presence of 1 mM CHAPS | Mus musculus | |
0.09 | - |
L-aspartate | - |
Mus musculus | |
5 | - |
glutamate | - |
Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Mus musculus | 5783 | - |
additional information | not in mitochondria | Mus musculus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | - |
- |
- |
Homo sapiens | Q8N9F0 | - |
- |
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mus musculus |
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
brain | - |
Homo sapiens | - |
additional information | In PCR, no specific amplification was observed with liver, kidney, lung, skeletal muscle, testis and heart cDNA of mice. | Mus musculus | - |
spleen | - |
Mus musculus | - |
thymus | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0015 | - |
activity of cloned mouse NAT8L fusion protein with N-terminal His6 tag | Mus musculus |
0.0018 | - |
activity of cloned mouse NAT8L fusion protein with C-terminal His6 tag | Mus musculus |
0.0025 | - |
activity of cloned wild type mouse NAT8L protein | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + glutamate | [14C]glutamate is acetylated with an approximately 50-fold lower affinity and a similar Vmax compared with aspartate | Mus musculus | CoA + N-acetylglutamate | - |
? | |
acetyl-CoA + L-aspartate | - |
Mus musculus | CoA + N-acetyl-L-aspartic acid | - |
? | |
acetyl-CoA + L-aspartate | - |
Homo sapiens | CoA + N-acetyl-L-aspartic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aspartate N-acetyltransferase | - |
Mus musculus |
aspartate N-acetyltransferase | - |
Danio rerio |
aspartate N-acetyltransferase | - |
Homo sapiens |
NAT | - |
Mus musculus |
NAT | - |
Danio rerio |
NAT | - |
Homo sapiens |
NAT8L | - |
Mus musculus |
NAT8L | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.56 | - |
N-acetyl-L-aspartate | - |
Mus musculus | |
6.1 | - |
glutamate | - |
Mus musculus |