Cloned (Comment) | Organism |
---|---|
expression of a truncated enzyme form lacking the transmembrane N- and C-termini in Escherichia coli as His-tagged protein, which is insoluble in absence of detergents | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics | Homo sapiens | |
0.00024 | - |
all-trans-retinol | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | integral | Homo sapiens | 16020 | - |
smooth endoplasmic reticulum | - |
Homo sapiens | 5790 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
1 * 25000, full-length wild-type enzyme, SDS-PAGE, 1 * 20000, recombinant truncated enzyme, SDS-PAGE | Homo sapiens |
20000 | - |
2 * 25000, full-length wild-type enzyme, SDS-PAGE, 2 * 20000, recombinant truncated enzyme, SDS-PAGE | Homo sapiens |
25000 | - |
1 * 25000, full-length wild-type enzyme, SDS-PAGE, 1 * 20000, recombinant truncated enzyme, SDS-PAGE | Homo sapiens |
25000 | - |
2 * 25000, full-length wild-type enzyme, SDS-PAGE, 2 * 20000, recombinant truncated enzyme, SDS-PAGE | Homo sapiens |
40000 | - |
nonreducing PAGE, higher aggregation, up to pentamers, occurs in absence of denaturing agents | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant truncated enzyme from Escherichia coli in presence of 1% SDS, by nickel affinity chromatography to homogeneity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
retina | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine + 11-cis-retinol | low activity | Homo sapiens | 2-acylglycerophosphocholine + 11-cis-retinyl acyl ester | - |
? | |
phosphatidylcholine + all-trans-retinol | - |
Homo sapiens | 2-acylglycerophosphocholine + all-trans-retinyl acylester | - |
? | |
phosphatidylcholine + retinol-[cellular-retinol-binding-protein] | - |
Homo sapiens | 2-acylglycerophosphocholine + retinyl-ester-[cellular-retinol-binding-protein] | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 25000, full-length wild-type enzyme, SDS-PAGE, 2 * 20000, recombinant truncated enzyme, SDS-PAGE | Homo sapiens |
monomer | 1 * 25000, full-length wild-type enzyme, SDS-PAGE, 1 * 20000, recombinant truncated enzyme, SDS-PAGE | Homo sapiens |
More | the enzyme exists as a mixture of monomer and dimer, determined by sedimentation equilibrium analysis and mass spectrometry, higher aggregation, up to pentamers, occurs in absence of denaturing agents | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
lecithin retinol acyltransferase | - |
Homo sapiens |
LRAT | - |
Homo sapiens |